Dna Fragment Ligation
Dna Fragment Ligation is a molecular biology technique used to join two pieces of DNA with complementary overhangs, by creating a covalent bond between the two strands. This reaction is commonly used in genetic engineering, to accurately and precisely insert an isolated DNA fragment into a vector, such as a plasmid. The two strands of DNA must have their ends compatible with each other, in order to form the ligation junction. The ligated DNA product can then be used in various downstream applications, such as expression and cloning of genes, for the study of protein structure and function, or for generating and studying gene libraries. Dna Fragment Ligation can be used to accurately and reliably construct engineered DNA sequences, essential for the progress of modern genetic studies.
← Journal of DNA And RNA Research