Primer Extension

Primer extension is a laboratory technique used to amplify a specific sequence of DNA, most commonly in the context of genotyping. It is also used in research to identify unknown polymorphisms and can be used in clinical settings for diagnosis. The technique is relatively simple to carry out and is usually done by adding a single stranded DNA primer at the end of the target sequence, followed by the extension of the primer with a DNA polymerase enzyme and the addition of a labeled nucleotide. The incorporation of the labeled nucleotide can be used to detect the size and sequence of the target DNA fragment. Primer extension is a useful tool for accurately and quickly identifying genetic variations that can have important implications in clinical research and applications.