Broth Microdilution

Broth microdilution is an in vitro method used to determine the minimal inhibitory concentration (MIC) of a given antibiotic against a microbe. It involves the addition of a serial dilution of the antibiotic to a liquid broth, followed by the addition of a standardized quantity of the test microorganism. The broth is then incubated at a specified temperature and time. After the incubation period, the MIC is determined as the lowest concentration of antibiotic at which no growth of the microbe is observed. The broth microdilution method is a reliable and preferred technique for comparing the susceptibility of different microbial species to antibiotics and other antimicrobial agents. Furthermore, it is an invaluable tool in epidemiologic studies, formulation of biocide products, and clinical trials.