Isothermal Titration Calorimetry

(ITC). Isothermal Titration Calorimetry (ITC) is a technique used to study biochemical processes and interactions. It is a powerful tool for analyzing the thermodynamics of reactions, such as binding affinity, as well as for understanding the energetics of processes such as drug-target interactions, DNA hybridization, protein-protein interactions and enzyme catalysis. ITC can also be used to measure the hydrophobic interactions of proteins and to determine the heat released when two molecules bind together. Its non-invasive and label-free nature make it an advantageous method for measuring thermodynamics in biochemical systems without perturbing the reaction.