Open Access Pub publishes peer-reviewed, free-to-read open-access articles. Showing
articles matching Protein. — open any to read the full text,
or download the PDF or XML.
Dec 2023 DOI 10.14302/issn.2691-6622.ijar-23-4637
M. Yagoub HassanCorresponding author
This study was conducted to evaluate the effect of socked pigeon pea seed meal on Growth and Food Utilization Efficiency of Nile tilapia (Oreochromis niloticus) fingerlings. 120 fish the weight and length average (3.4±0.04g and 3.2±0.00cm) were randomly distributed in 12 plastic Tanks (Aquarium) 36L according to the complete randomization, allocated to a represent four treatments (T0, T1, T2, and T3) of Nile tilapia (Oreochromis niloticus) fingerlings. four feeds were prepared in pellet form which consisted of 0%, 25%, 50% and 75% pigeon pea seed meal as replacement of fish meal. All diets contents 30% protein. Nile tilapia (Oreochromis niloticus) fingerlings were fed at the rate of 5% of their body weight. The result showed significant variation (P < 0.05) in final body weight, weight gain (WG), daily weight growth (DWG), Specific Growth Rate (SGR), and Food Conversion Ratio (FCR) among treatments. But, high SGR in T0 (control) and the best FCR were obtained at T 0% pigeon pea seed meal. Over all, best growth performance was observed in fish fed diet T1 (25%). The best performing fish’s final weight, DWG, SGR and FCR were 9.46±0.33g, 0.13±0.01g/day, 2.93±0.73% /day and 1.49±0.30 respectively. On the other hand, there was high significant variation (P < 0.05) in survival rate were obtained at T2 (50%). this study recommend that a diet containing 25% Pigeon pea seed meal for juvenile Nile tilapia is optimum in terms of growth performance and profitability, and does not impact water quality and plankton abundance in plastic Tanks (Aquarium) culture system.
Apr 2022 DOI 10.14302/issn.2372-6601.jhor-22-4133
Qing XinCorresponding author
Department of Pathology, Harbor-UCLA Medical Center, 1000 West Carson Street, Torrance, CA 90502, USA.
Background Monoclonal gammopathy of undetermined significance (MGUS) and chronic myeloid leukemia (CML) are diseases of different lineages. The diagnosis of both MGUS and CML in the same patient is a rare occurrence and has not been reported in much literature. Case Presentation We describe a 56-year-old man with a history of rheumatoid arthritis incidentally found to have an increase in IgA paraprotein. With less than 10% monoclonal plasma cells on the bone marrow biopsy and absence of hypercalcemia, renal failure, anemia and bone lesions, MGUS was diagnosed. The conventional cytogenetics at the time showed the presence of the Philadelphia chromosome in 30% of metaphases. However, there was no morphologic evidence of CML in the peripheral blood or bone marrow. Patient received no treatment and lost follow-up until 3 years later when a routine CBC showed leukocytosis and thrombocytosis. CML, chronic phase was diagnosed following a bone marrow aspiration and biopsy with Philadelphia chromosome observed in 100% of metaphases. Patient was treated with imatinib and later switched to dasatinib and complete molecular remission was continued to be achieved. Discussion and Conclusion Here we report a case of pre-leukemic CML as an incidental finding during the diagnosis of MGUS. The possible underlying mechanisms of the association are discussed although the exact cause of the coexistence is unclear.
Nov 2020 DOI 10.14302/issn.2691-6622.ijar-20-3605
Nsangou Amidou KpoumieCorresponding author
Department of Animal Sciences, University of Dschang, Cameroon
In order to contribute to the domestication of Labeobabusbatesii, the survival and growth performances in pre-grossing according to the dietary protein level were studied between January and April 2020. A total of 400 fry (0.8 ± 0.02g), divided into four batches and distributed randomly in 8 happas (50 ind / m2), respectively received in duplicate the food at 25, 30, 35 or 40% crude protein (R25%, R30%, R35% and R40%). The results show that: survival rate and growth performances were significantly influenced (p≤0.05) by the level of dietary protein except for lengths (total, standard and length gain) whose highest values were obtained with the diet at 35% crude protein, the highest significant values of the other growth characteristics were obtained in the batch fed at 25% crude protein while the lowest were recorded on individuals of batches fed at 40% crude protein. Regardless of the level of food protein, the post-larvae presented an allometric minorant growth. So, 25% of dietary protein is therefore recommended for pre-grossing of this species.
Aug 2020 DOI 10.14302/issn.2474-7785.jarh-20-3425
Jana SnehasisCorresponding author
Trivedi Science Research Laboratory Pvt. Ltd., Thane (W), Maharashtra, India.
The study was aimed to investigate the potential benefits of the Consciousness Energy Healing Treatment (the Trivedi Effect®) per se and Biofield Energy Healing treated novel test formulation in male Sprague Dawley rats for their antiaging activity by monitoring aging biomarkers such as brain-derived neurotrophic factor (BDNF), silent information regulator-1 (SIRT-1), and klotho protein. The test formulation was distributed into two parts. First part did not provide any Biofield Energy Treatment was denoted as the untreated sample, however the second part was received Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi and defined as the Biofield Energy Treated sample. In this experiment, nine groups (n=10) were assigned, in which four were preventive maintenance groups. Among them, three groups of animals were also received Biofield Energy Healing Treatment per se (at day -15). BDNF was significantly increased by 25.83%, 19.35%, and 14.67% in the Biofield Energy Treated test formulation (G5), Biofield Energy Treatment per se at day -15 (G6), and Biofield Energy Treatment per se to animals plus Biofield Treated test formulation from day -15 (G8), respectively as compared to the disease control (G2) group. Moreover, expression of SIRT-1 protein was increased by 14.63% in the G5 group than the untreated test formulation (G4) group. Additionally, SIRT-1 activity was increased by 39.7%, 32.5%, 15.9%, and 136% in the G6, Biofield Energy Treated test formulation at day -15 (G7), G8, and Biofield Treatment per se (day -15) to animals plus untreated test formulation (G9) groups, respectively than the G4 group, while it was increased by 57.3% in the G9 group as compared to the G2 group. Besides, Klotho protein in kidney homogenate was significantly increased by 16.67% in the G5 group as compared to the G2 group. Altogether, the results showed a significant improvement of longevity mediators and antiaging biomarkers in the preventive maintenance groups. Therefore, results envisaged the significant slowdown of aging-related disorders and other complications in the preventive Biofield Energy Treatment group per se and/or Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) comparatively with the disease control group and could be utilized against various aging-related disorders like Alzheimer's disease, hypertension, osteoporosis, cataracts, type 2 diabetes, cancer, etc. along with it could be used to extend the life-span, stress and immune-related disorders.
Apr 2020 DOI 10.14302/issn.2691-8862.jvat-20-3278
Isea RaúlCorresponding author
Fundación Instituto de Estudios Avanzados, Hoyo de la Puerta, Baruta. Venezuela.
The goal of this paper is to obtain the numerical consensus of B cell epitopes from the three-dimensional structure of the prefusion spike glycoprotein of the new betacoronavirus that could lead to the development of a vaccine to 2019-nCoV. In order to do that, we first calculated the B-cell epitopes that are predicted using fourteen different mathematical algorithms. Later, we obtained the consensus of B-cell epitopes according to the Similarity Index, and finally selecting the best candidates according to the results of a function called <F> which is evaluated for the glycoprotein. The best candidates that we obtained in order to design a vaccine are SSANNCT, PLQSYGFQPT, TESNKKFLP, NNSYEC, AENS, LPDPSK and YDPLQPE.
Jan 2020 DOI 10.14302/issn.2379-7835.ijn-19-3139
Q. Almulaiky YaaserCorresponding author
Chemistry Department, Faculty of Sciences and Arts, University of Jeddah, Khulais, P.O. Box 355, Khulais, 21921, Saudi Arabia
In this study, a peroxidase from new source was purified using ion exchange and gel filtration techniques. The recovery for peroxidase activity was 19% with 11-fold purification and specific activity of 749 unit/mg protein. Purified peroxidase demonstrated a molecular mass of 39 kDa using gel filtration and was confirmed as a single band on SDS-PAGE. The purified peroxidase revealed a broad optimum pH activity at 6.0-6.5 and 50°C temperature. The kinetic parameters for purified peroxidase toward H2O2 and guaiacol as substrates were found to be Km = 3.355, 5.395 mM, Kcat = 99.52, 79.56 s-1 and Vmax =1.531, 1.242 µmole ml-1 min-1, respectively. The catalytic efficiency (kcat/Km) of the purified peroxidase was 14.75 and 29.66 s−1 mM−1 for guaiacol and H2O2, respectively. Peroxidase activity was observed to be enhanced by Cu2+, Co2+, Ni2+ and inhibited in the presence of Sn2+, Al3+, Hg2+, NaN3, EDTA and urea. Characterization showed that peroxidase purified from C. forskohlii has the ability to be used for food industrial applications.
Mar 2019 DOI 10.14302/issn.2471-2140.jaa-19-2699
M.M. Masoud HassanCorresponding author
Molecular Biology Department, National Research Centre, El-Tahrir st., Dokki, Giza, Egypt.
Xanthine oxidase is a commercially important enzyme with wide area of medical applications to develop diagnostic kits. Xanthine oxidase was extracted, purified and characterized from sheep liver (SLXO). The purification procedure involved acetone precipitation and chromatography on DEAE-cellulose and Sephacryl S-300 columns. The sheep liver xanthine oxidase was homogeneously purified 31.8 folds with 3.5 U/mg specific activity and 24.1% recovery. SLXO native molecular weight was 150 kDa and on SDS-PAGE appeared as single major band of 75 kDa representing a homodimer protein. Isoelectric focusing of the purified SLXO resolved into two closely related isoforms with pI values of 5.6 and 5.8. The apparent Km for xanthine oxidase at optimum pH 7.6 was found to be 0.9 mM xanthine. FeCl2 and NiCl2 increased the activity of SLXO, while CuCl2 and ZnCl2 were found to be potent inhibitors of the purified enzyme. Allopurinol inhibits SLXO competitively with one binding site on the purified molecule and Ki value of 0.06 mM.
Aug 2018 DOI 10.14302/issn.2379-7835.ijn-18-2212
T NkrumahCorresponding author
Department of Animal Science, Faculty of Agriculture, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Fish meat is a common and broadly used food due to its high nutritional values yet the bones and flavour of fresh fish can be unpleasant. The unpleasant fishy flavour makes it difficult to handle and process. The fishy flavour is as a result of microbial presence and growth. Studies associated with microbial contaminations have concentrated more on the carcass. Such contaminations which affect the microbiological status of processed products can emanate from spices and other non-meat ingredients, environment, equipment and handlers. The successful application of processing/preservation technology results in the conservation of desirable qualities in stabilized and varietal fish products. This study sought to use fish in the manufacture of frankfurter-type sausages, which could have improved preservation characteristics without any adverse effects on sensory properties. The nutritional composition and microbiological safety of fresh fish and sausages were determined using the methods described by AOAC and ICMSF respectively. The study showed that, catfish sausages were higher in protein (15.69 %) and were lower in fat (10.66%) compared to the other sausages. Total Viable Counts (TVC) were within the accepted limits (106 and 107cfu/g) for fish and pork respectively. E. coli was not detected in any of the treatments during frozen storage for 6 weeks. It was concluded that catfish frankfurter has high nutritive value because it contained less fat but with higher crude protein. Like pork frankfurters, both catfish and mackerel sausages could be stored for six weeks without any negative effects on microbial quality.
Apr 2018 DOI 10.14302/issn.2326-0793.jpgr-18-2004
Anwar PervezCorresponding author
Department of Biochemistry and molecular Biology, University of Gujrat Sialkot subcampus, Pakistan
Human proteome project was revolutionized about 40 years ago with purpose of summarizing whole proteomic data at one place. It was launched after human genome project to map and observe all proteins. The goal related proteomic study is to draft the entire human proteome in disease diagnosis by using bioinformatics tools. Pillars of human proteome project provide different databases related to proteins at transcriptional and translational level. Human proteome organization(HUPO) published biology disease HUPO whose aim is to measure protein and proteome by life and processes related to human diseases. Different human organ like plasma, liver, brain and diabetic base project are used to characterize human disease and health. Major data resources accumulated in databases like peptides Atlas, GPMDB and neXtProt for proteins. Matrices of human proteome project identify and characterize the protein products as Post translational modification (PTM), splice various isoforms from 20,300 proteins. Matrices related to different years make proteomes counterpart by magnify the research biomedical community with high output of instruments and specimen pre-analytical protocols. CALIPHO multidisciplinary group provides information about protein complexities, interactions, function and structure complexities after Uniport and Swissprot. Different bioinformatics tools are used for structural and functional annotations of protein, disease diagnosis and mutations due to protein. Extensive study of human proteome project has been proved helpful in disease treatment at translational and post- translational levels. In future, human proteome project along with bioinformatics will include protein profiling, biomarkers, Mass spectrophotometer technique and cross analysis of different proteome projects.
Jul 2016 DOI 10.14302/issn.2576-2818.jfb-16-1035
Sugimoto MayumiCorresponding author
National Livestock Breeding Center, Nishigo, Fukushima, Japan,
Female fertility is an economically important trait in the dairy industry, and the fertility selection index has been developed as a method of including female fertility in the breeding goals of this industry. This index considers a combination of factors, including days open, number of inseminations per lactation, success after first insemination, and pregnancy within 70 d, 90 d, and 110 d after parity. Based on a genome-wide association study of the fertility selection index using 442 Holsteins, we found that the index is influenced by a variation in the thioredoxin fold region of the family with the sequence similarity 213, member A (FAM213A) protein. FAM213A is a CXXC motif-containing peroxiredoxin 2-like protein that regulates cellular redox status. A replacement of isoleucine with valine in FAM213A was associated with poor fertility in cows. The overexpression of FAM213AVal in bovine endometrial epithelial cells reduced reactive oxygen species to a lesser extent relative to the overexpression of FAM213AIle and caused a decrease in cyclooxygenase-2 expression. Downregulation of cyclooxygenase-2 led to a decline in prostaglandin E2, which is critical for implantation because it protects the conceptus from the maternal immune system. Cows with FAM213AVal showed lower levels of prostaglandin E2 than did cows with FAM213AIle, suggesting that cows with FAM213AVal are less fertile than cows with FAM213AIle because of their reduced uterine environment. Thus, the present study found that FAM213A unexpectedly modulates female fertility in cattle.
Jun 2015 DOI 10.14302/issn.2379-7835.ijn-14-479
Erdmann JohannesCorresponding author
Department of Nutritional Medicine, University of Applied Sciences Weihenstephan-Triesdorf, Weidenbach, Germany
Objective: Detailed reports in changes of eating habits especially in elderly subjects with successful weight loss and maintenance are virtually unknown. In this paper we have analyzed the eating habits of 104 obese patients (BMI 37.8±0.3 kg/m2, age range 65 and 84 years) who lost 11.5±0.3kg over an average follow-up period of 37.5±0.5 months. Design: Patients recorded food intake over a period of 12 days before and 12 days after changes of eating habits. Dietary counseling was based on food energy density (ED) with the aim to maintain food quantity as much as possible for adequate satiety in conjunction with a reduction of energy intake. Individual eating habits should be preserved as much as possible. Results: Average daily energy intake of solid food was reduced by 267kcal, food intake by 114g and ED by 0.10 kcal/g, respectively. Liquid calories were reduced by 79 kcal/d. Lower daily energy intake was the result of a reduction of medium and high ED food items compensated for by a greater intake of low ED food items. Changes of macronutrients comprised substantially lower carbohydrate and fat intake with minimal reduction of protein. From 28 different food groups 9 were reduced significantly (bread, butter, marmalade, cheese, meat products, fast food, fruit and chocolate) while 3 were increased (eggs, curds, ham). The relative contributions of the various food groups to daily energy intake remained fairly similar indicating that individual preferences were largely maintained. Conclusion: The data demonstrates that in elderly subjects with severe obesity an individual change of eating habits can lead to successful long-term weight loss with improvement of carbohydrate metabolism. This will contribute to increased mobility and to improved quality of life. This method which is based on the energy density of food items is a simple measure to reduce energy intake while ensuring long-term adherence.
Jun 2014 DOI 10.14302/issn.2328-0182.japst-13-288
Pradhan N.Corresponding author
Sr. Professor and Head, Department of Psychopharmacology, NIMHANS, Bangalore, INDIA, 560001
ALS is the neurodegenerative disease which is caused due to breakdown in interaction between UBL and rpn1. In this study, we explore the interaction of UBL and rpn1 which is involved in protein degradation. Protein recycling system plays a crucial role in degradation of deformed or damaged proteins. Task of degradation of damaged ubiquitinated proteins is completed by proteasome with the help of ubiquilin2 protein which links 19s proteasome and poly-Ub chain attached to damaged protein. More specifically, N-terminal UBL domain interacts with rpn1 subunit of base complex of 19s proteasome and C-terminal UBA domain interacts with tetra poly-Ub chain attached to damaged protein. In present study, UBL domains are docked against homology modeled rpn1 with the help of Patch dock server. Further the docked structures are refined using fire dock server and best docked structure is chosen having global energy -16.71. Best docked structures are analyzed using swiss-pdb viewer software to show hydrogen bonds between interacting proteins. Here we explore a mutation E6A and P11A in UBL structure with the help of YASARA which is significantly increasing the interaction between interacting proteins in terms of hydrogen bonds.