Search results for “experiment”

Exploring the Mechanism of Complex Lemon-Angelica Sinensis-Boswellia Essential Oil on Anxiety Disorders with Melasma Through Network Pharmacology and Experimental Validation

The incidence rate of melasma is notably high among patients with anxiety disorders. Aromatherapy primarily influences the physiological and psychological states of individuals through the inhalation or application of essential oils, thereby facilitating the treatment or alleviation of various conditions. This study aims to explore the action mechanism of complex lemon-angelica sinensis -boswellia essential oil (CEO) in treating anxiety disorders with melasma. We investigated the active ingredients, targets, and pathways of CEO in relation to anxiety and melasma using network pharmacology. We employed cell assays and conducted nebulized essential oil inhalation tests on CUMS mice to validate the intervention effects of CEO on anxiety. A total of 28 active components, including neryl acetate, 3-butenylphthalide and octyl acetate, and 26 cross-targets, such as ESR1, CCND1 and PIK3CA, were identified. GO and KEGG pathway analyses indicated that these cross-targets were primarily involved in endocrine regulation, cell proliferation, and apoptosis, specifically through PI3K/Akt signaling pathway and calcium signaling pathway. The experimental results demonstrated that CEO significantly reduced the secretion of NO, TNF-a and IL-6, as well as the mRNA expressions of ESR1, CCND1 and PIK3CA in cells compared to the inflammatory cell model. Furthermore, CEO notably decreased the forced swimming immobility time of mice and the levels of IL-1β, ESR1 and CCND1 in hippocampus when compared to model mice. These findings suggest that CEO may regulate ESR1, CCND1 and PIK3CA through its citral, 3-butylphthalate and neryl acetate, thereby influencing endocrine function, cell proliferation and apoptosis, inhibiting inflammation and anxiety-like behavior in CUMS-induced mice.

A Review of Attempts to Identification and Antifungal Susceptibility of Dermatophytes (Microsporum Canis and Tricophyton Mentagrophytes) Isolated from Infected Cats and Dogs with Experimental Dermatophytosis of Guinea Pigs

Dermatophytosis affect companion animal’s skin and keratin appendages as cats and dogs, resulting in red, scaly, itchy, bald, and raised patches like ring. The three main groups are Microsporum, Trichophyton and Epidermophyton. This study collected samples of skin scrapping and hairs from 130 cats and 70 dogs, using common mycological approach samples were examined. Antifungal agar disc diffusion and broth microdilution assays were utilized on some of the isolates. Three groups of Guinea pigs (6 in each) were then infected with one isolate of M. canis or T. mentagrophytes fungi, another skin scrapping samples of virulent fungi was isolated on the 7th and 14th days, blood samples were collected at 14th day. Reverse transcription-PCR to detect 98 bp protease gene. Resulting in 45% of cats and dogs tested positive for Microsporum and Trichophyton species. Agar disc diffusion revealed that the antifungal medication griseofulvin was the most effective against tested isolates. The best results for MIC test were griseofulvin (0.98 µg/ml) followed by acetic acid (0.28 µg/ml). Differential leukocytic count of Guinea pigs showed that monocyte levels remained unchanged, while neutrophil and lymphocyte levels had increased. The active (isolates from Guinea pigs skin scrapping) and dormant cells (isolates from keratin free media) were distinguished by Reverse Transcriptase-PCR. Collectively, qPCR is a successive and feasible method for the diagnosis for Microsporum and Trichophyton species.

Baroreflex Sensitivity, Cardiac and Kidney Remodeling and Deterioration in Vasoactive Substances Content in Blood in Experimental Model of Renovascular Hypertension. Action of Natural Flavone, Luteolin

This study aimed to analyze pharmacological actions of phenolic compound luteolin on the renal and cardiac hypertrophy, blood pressure (BP), baroreflex sensitivity (BRS), levels of epoxyeicosatrienoic acids (EETs), prostaglandin E-2 (PGE-2) and endothelin-1 (E1) in plasma in the 2 kidney - 1 clip (2K-1C) model of renovascular hypertension (RVH). All animals, were randomized into 2 groups: control (normal) I - sham-operated, II- RVH male Wistar rats, which after 4 weeks of surgical intervention secondly randomized to control II group, treated 0.1% dimethyl sulphoxide (DMSO) and main group - with luteolin in 15 DMSO, 3 mg/kg body weight, intraperitonially, during 2 weeks. ET-1, EETs and PGE2 levels investigated in carotid artery blood plasma and analyzed using ELISA kits. All data statistically analyzed using the SPSS-10.0 program. In RVH rats BP increased by 32%, cardiac and right kidney hypertrophy and reduction in parasympathetic component of BRS by 40% and sympathetic by 39%. The plasma level of total trans-EETs and PGE2 in RVH rats decreased by 44% and 50% respectively, while the level of ET-1 increased by 67%. Two weeks treatment with luteolin lowered BP, improved parasympathetic, without marked changes in sympathetic component of BRS. Deremodeling of cardiac and renal hypertrophy under prolonged treatment with luteolin accompanied with increasing in the level of EETs by 44%, PGE-2 by 50% and markedly reducing of plasma content of ET-1 (by 60%). Inhibition of EET hydrolase using low doses of luteolin provides beneficial cardio and renoprotective action in experimental model of RVH.

Experimental Study by the Plan Composite Centered Method, Modeling and Optimization of Hydrogen Production by Alkaline Electrolysis

A planned experimental study on the production of green hydrogen by alkaline electrolysis is carried out by the Plan Composite Centered (PCC) method. The parameters studied are the concentration of the electrolyte, the distance between electrodes, the height of the electrodes, the total supply voltage of the electrolyser, temperature, and the electrolyte type. The results show that the effect of concentration, height, voltage and temperature are positive. However, the effect of the distance between the electrodes is negative. Electrolysis with potassium hydroxide (KOH) is more efficient than with sodium hydroxide (NaOH). The second-order interactions are weak, except for the voltage-temperature interaction which is significant. The results of the experimental study conducted in this work are in agreement with previous studies. Two a polynomial modeling (with KOH and with NaOH) suitable for predicting the flow of hydrogen produced are presented. Three optimizations of ascending constraints on the operating parameters to have a maximum hydrogen production and with a minimum of electrical energy and a minimum of concentration consumed are carried out.

Evolution of the Solid Human Tumor Cells Properties in Various Experimental Systems in Vitro

Background Human malignant cell models which reflect the structural and physiological complexity of tumor tissue are of great importance for preclinical research in oncology. Spheroids/tumoroids derived from solid tumors are of great interest as cellular models mimicking the first vascular-free growth phase of a tumor node. The fact of the identity between artificially created tumor multicellular aggregates and the real tumor tissue, however, needs to be specified, described and validated in order to see how closely the spheroids are biologically similar to the malignized tissues in vivo compared to the monolayer cell cultures traditionally used. We present here a comparison study of the characteristics of solid tumor cells of different histogenesis (melanomas, soft tissue sarcomas and bone sarcomas, epithelial tumors) cultured in two dimensions (monolayer culture) and three dimensional space (spheroid), namely: spatial organization, multiplication, metabolic activity. Patients and Methods For the creation of 2 D and 3D cell models the cells isolated from the patient's solid tumor fragments obtained intraoperatively were used. 15 samples of skin melanoma, 20 samples of soft tissue and osteogenic sarcomas (STBS), and 9 samples of epithelial tumors (ET). The tumor cells were all cultivated for at least 10 passages. We used phase contrast, confocal microscopy, and immunohistochemistry to investigate spheroids and monolayer cultures. The supernatants of tumor cells grown in 2D and 3D cultures were studied using ELISA and multiplex analysis for the production of a spectrum of chemokines and cytokines supporting the immunosuppression, invasion and metastasis processes. Results Tumor specimens received were predominantly of metastatic origin (75%). In 100% of cases 2D cultures were received, in 88.6% of cases (39 out of 44) we succeeded in obtaining spheroids. There was no direct correlation between the efficiency of tumoroid formation and the tumor's histogenetic origin and the stage of the cancer process (primary tumor, recurrence, metastasis). The median size of spheroids by 4-5 days of cultivation with a starting concentration of 10000 cells per well was 657.14 μm for melanoma (min 400 - max 1000 μm), 571.42 μm (min 400 - max 700 μm), 507.14 μm (min 300 - max 600 μm) for soft tissue sarcomas, 650.0 μm (min 400 - max 900 μm) for osteogenic sarcomas. Immunochemical analysis of Ki-67, GLUT1, and Ecadherin markers was carried out for tumor tissue samples, single-layer tumor cultures, and tumoroids of every patient. The distribution of the stained groups in the spheroids was distinct from the monolayer cultures and more in accordance with the distribution of such in the tissue tumor, the number of Ki-67+ cells was increasing in the spheroids. We detected no dependence of Ki-67+ and GLUT1+ cell localization grade on spheroid size. We identified E-cadherin in tumor tissue and tumoroids of breast carcinoma and one melanoma culture. Monolayer cultures did not express it. The increase in secretory cell activity of the solid tumor cells from 2D to 3D system was observed when CCL2, CCL3, CXCL1, CXCL16, MIF, IL10, MICA (p<0.01) were investigated. Conclusion The presence of patient-specific cells of solid tumors in a 3D environment causes activation of the proliferative and metabolic processes as compared to monolayer cultures, which makes these models approximate the real world clinical picture. The production of chemokines that can attract to the tumor various types of immune system cells, to include their immature versions, as well as production of cytokines and Immunosuppression factors that, when present in the tumor microenvironment in the high concentrations, contribute to the formation of immune cells having suppressive capacities occurs in the 3D cell system. Three-dimensional model of the initial tumor nodule formation stage thus demonstrates the forming process of tumor cells favorable for them microenvironment. Construction of three-dimensional models - spheroids of tumor cells of differing histogenesis demands individual approach and more thorough investigation.

Culture of Cyclops for Use the First Intermediate Host in Experimental life Cycle of SpirometraSpecies

Background In natural conditions Cyclops are the first intermediate hosts in the life cycle of Spirometra species. In this paper we describe simple method of culturing Copepod of the genus Cyclops for use the first intermediate host in experimental life cycle of Spirometra species. Methods Paramecium was first cultured to be used as food for Cyclops. Sample of water was collected from a pond within Sokoine University. About 100 ml of water and pre-boiled wheat grains were transferred in a Petri dish and kept under laboratory conditions for 7 days, a swarm of Paramecium was formed. An adult female egg sacked Cyclops from a natural water pond in Tarangire National Park, Tanzania was added in a new Petri dish containing tap water, 0.3 ml of Paramecium suspension and 4 pre-boiled wheat grains. The mixture was kept under laboratory conditions temperature 26-29⁰C and observed daily. Results Eggs from the single Cyclops hatched to nauplius. The average time of developing to nauplius I was 1.2 days, nauplius I to copepodite I was 6.9 days, and copepodite I to adult female Cyclops was 26.3 days. The average measurements of naupliusI were 120.2µm length and 80.0µm width while the adult female was 846.3µm length and 284.6µm width. The adult female produced 1 to 8 broods (mean 4.3). The life span of Cyclops averaged 43.1 days. Conclusion The Cyclops cultured in the laboratory were fed Paramecium and used as first intermediate host in the experimental life cycle of Tanzanian Spirometra species.

Experimental Analysis PVP Coated Silver Nanofluid Properties for Application in Photovoltaic/Thermal (PVT) Collectors

Nowadays, supplying energy for the global population has turned into a prominent issue for countries engendering the consumption of huge amounts of fossil fuels which leads to some serious environmental problems. Among the renewable energy technologies, solar collectors can play major role to improve the efficiency, in air conditioning utility systems by minimum pollution. In photovoltaic/thermal (PVT) solar collectors, which are currently considered as the most advanced type to produce electricity and heat simultaneously, working fluid absorbs Energy from photovoltaic (PV) module engendering to decrease temperature of PV module and increase the electricity efficiency and also provide permissible amount of heat for other residential applications. Meanwhile, utilizing nanofluid as the working fluid in collector, regarding that the nanofluid has enhanced thermal properties relative to the base fluid, leads to a higher collector efficiency. In this research, PVP coated silver nanofluid was prepared in three volume concentration being 250, 500 and 1000 ppm by two-step method. To assess the stability of nanofluid the zeta potential is calculated which is obtained -41.6 V. Also, the prominent thermal properties of the nanofluid were analyzed regarding PVT solar collector applications. According to the results, thermal conductivity of the PVP coated silver nanofluid, improves the properties of base fluid, to the extent that thermal conductivity coefficient grows up 50% in some temperatures and increased from 0.594 for base fluid to 1.098 W/mK by escalation of concentration to 1000 ppm. Thus, PVP coated silver nanofluid can be deemed as the vital working fluid to improve the performance of PVT solar collectors.

Therapeutic Evaluation of Neemazal® Against Experimental Eimeria Tenella Infection in Broiler Chickens, Jos - Nigeria

Coccidiosis in poultry is caused by protozoan parasites of the Eimeria species, which is responsible for worldwide economic losses. The aim of this study was to evaluated the therapeutic effect of NeemAzal® on Eimeria tenellain broiler Chickens as compared to Amprolium as a standard anticoccidial drug. A total of One Hundred and Sixty (160) broiler chicks were purchased, acclimatized and randomly divided into 4 groups (G1, G2, G3 & G4). G1 non-infected, non-treated (negative control), (G2) infected with 20000 E. tenella oocysts (positive control), (G3) infected and treated with Amprolium (Standard, 7 mg/kg b.w. for 5 days) and (G4) infected and treated with NeemAzal®200 mg/kg b.w. for 5 days). Evaluation was by clinical signs, performance data (weight gain, oocyst shed/gram faeces (OPG) and histopathology of the Caecum, Liver and Kidney. The data showed that birds infected with E. tenellahad an output of 1.3×105±3,333 oocysts per gram faeces on day 5 post inoculation. This output is significantly decreased to 0.37×105±3,111 oocysts in neem-treated birds. Infection with E. tenellainduced marked histopathological alterations in the caecum in the form of inflammation, vacuolation of the epithelium, and destruction of some villi. NeemAzal® decrease body weight loss of infected chickens. Moreover, the number of goblet cells stained with Hematoxylin and Eosin (H&E) within the infected villi was significantly lowered (P≤0.05). The results revealed that chicks of G1 had the best performance data compared to G2, G3& G4. In G3 & G4 there were a remarkable improvement in the data on performance, clinical signs, gross and microscopically caecal lesions compared to G2. Amprolium (G3) was shown to be superior to NeemAzal® (G4) compared to G2. NeemAzal® could be a good alternative for use as a coccidiostat to supplement the expensive anti-coccidiostats in the market.

Abelmoschus Esculentus (Moench.) Seed Derived Flavonoids Antioxidant Protect the Heart Against Experimental Oxidative Injury

Since oxidative stress impairs the cardiovascular function, the hypothesis from the present study is that the treatment of paraquat-exposed adult Wistar rats with methanolic extract of Abelmoschus esculentusseed would reduce paraquat-induced cardiovascular damage. Thirty healthy female Wistar rats weighing 120-150 g were randomly assigned into 6 groups of 5 rats each (Groups A, B, C, D, E and F). Rats in groups A served as control and received normal saline while groups B, C, D, E and F received a single dose of paraquat (7mgkg-1i.p.). Rats in group B was sacrificed 24hours following paraquat administration while daily administration of 100 mg kg-1and 200 mg kg-1 of methanolic extract of Abelmoschus esculentusseed extract were given orally to groups C and D while group E received daily oral dose of Vitamin E at 100mgkg-1 and group F was left untreated. Histological and biochemical preparations of the heart was made and data were expressed as mean± SEM. Significant difference was set at p<0.05. Results showed no significance difference (p<0.05) in nitric oxide activity, Glutathione reductase activity, and troponin I activity across the paraquat-exposed groups when compared with control. Histological studies reveal distortion of normal cardiac histo-architecture in paraquat-exposed group B compared with control rats while Abelmoschus esculentus reversed these changes in other treated groups. The study concluded that paraquat caused significant distortion of the cardiac histo-architecture and methanolic extract of immature Abelmoschus esculentus seed had antioxidant and ameliorative effects similar to Vitamin E on paraquat-induced myocardial injury.  

Reproductive Performance and Factors Influencing Fetal Outcome: A Practical Guide to Experiments on Lung Development in A Nitro Fen-Induced Rat Model for Congenital Diaphragmatic Hernia

Our study aims to help researchers calculate resources based on a rat breeding colony for experiments on fetal lung development. The Wistar rat is commonly used in experimental research. In the context of fetal lung studies, the nitrofen rat is a model for pulmonary hypoplasia and congenital diaphragmatic hernia. Data needed to calculate resources for new experiments are not easily available. We prospectively acquired data on the reproductive performance and fetal outcome of 314 consecutive virgin Wistar dams in our fetal lung research breeding colony. We define the impact of breeding conditions on rat fertility and evaluate different methods to diagnose early rat gestation. Effects of nitrofen exposure as well as fetal surgery in terms of prenatal mortality and gross anatomical parameters of lung development are quantified in nitrofen-exposed and -unexposed fetuses from embryonic day 19 to 21 in 12h intervals. A reduced mating interval (1h) provides a great degree of experimental control with feasible pregnancy rates and a large litter size. Nitrofen exposure, as well as fetal surgery, depict high fetal survival rates. Fetal lung findings were very reproducible and could allow for reduction of animals utilized within experimental groups.

Experimental-Morphological Study of Reparative Bone Formation with The Replacement of The Cavity Defect of The Bone with A Mesh of Titanium Nickelide

Purpose of Study To study reparative osteogenesis and tissue integration characteristics for implanting three-dimensional mesh structures of titanium nickelide into a bone cavitary defect. Material and Methods The authors modeled cavitary defects of femoral metaphysis experimentally in Wistar rats divided into an experimental group and control one. The study duration was 60 days in total. The methods of radiography, those of light and electron microscopy, X-ray electron probe microanalysis used. Results Under implantation the defect was filled with cancellous bone the volumetric density of which more than 1,5-fold exceeded control values (р < 0.001). The implant had biocompatibility, osteoconductive and osteoinductive properties, it stopped inflammatory processes. The membrane protective barrier which prevented connective tissue sprouting was formed on the implant surface in the defect periosteal zone. The osteointegrative junction was formed being persisted up to the end of the experiment. Reparative osteogenesis was performed by direct intramembranous and apposition type. Conclusion The implant of three-dimensional mesh titaium-nickelide structures has marked osteoplastic properties, and it can be successfully used in orthopedic surgery.

Experimental Zoology. Advancements, Problems and Future Prospects

The Antidepressant-Like Activity of the New Tetrapeptide Neuroprotector Kk-1, Homologous of Acth15-18 Sequence (An Experimental Study)

Theaim of study is to evaluate antidepressant-like activity of the new peptidergic neuroprotectoracetyl-(D-Lys)-Lys-Arg-Arg-amide, homologous of ACTH15-18 primary amino acids sequence, that demonstrates nootropic and neuroprotective properties. Using Porsolt swimming test (PST) efficacy of tetrapeptide neuroprotector KK-1 at a single dose of 0.02 mg/kg was investigated on 16 white random bred male rats (body mass equaled 180-220 grams). Imipramine (15 mg/kg i.p.) was used as a reference drug. Then depression was induced in these rats by reserpine (4 mg/kg, i.p.). The KK-1 (intranasally, i.n.) and imipramine were administered once a day during 3 days until the reserpine-induced depression was reproduced. The indices of rats behavior under the conditions of open-field test (OFT) and PST were evaluated. The influence of both drugs on specific reserpine-induced depressions symptoms (hypothermia and blepharoptosis) was also registered. The results were processed statistically. The tetrapeptide neuroprotector KK-1 reduced immobilization time of rats at PST (statistically significant differences compared with control group), exceeding efficacy of reference drug imipramine. Normalizing of locomotor and exploratory activity in the OFT, decreasing indices of rats helplessness behavior in PST by tetrapeptide neuroprotector KK-1 demonstrates its antagonism with depressive action of reserpine. The tetrapeptide KK-1 showed antidepressant-like action both in intact rats and in rats with the model of reserpine-induced depression. It reduced specific symptoms of depression – hypothermia and blepharoptosis, exceeding the activity of reference drug imipramine.

Vibrational, Ultra Violet, Natural Bond Orbital Analysis of E-1 using Quantum Mechanical Computations and Experimental Spectra.

The FT-IR, FT-Raman and UV-Vis spectra of E-[1-(3'-methylthienyl)-5-Phenyl-2,4-Pentadiene-3-one (MPPO) were recorded. The optimized molecular bond parameters, harmonic frequencies were calculated using B3LYP method with 6-311++G (d,p) basis set.The various normal modes were precisely assigned with thehelp ofTED calculation. The theoretical spectrograms for FT-IR, FT-Raman and Ultra Violet visible. Spectra of the title molecule had been constructed. The ICT was calculated by means of Natural Bond Orbital analysis. The Non Linear Optical properties related to polarizability and hyperpolarizability based on the finite-field approach were calculated.The band gap energy was calculated using HOMO-LUMO analysis. Furthermore, the Molecular Electrostatic Potential, Mulliken atomic charges and thermodynamic properties of MPPO were also calculated.

Mucosa-Muscular Signaling for Bile-Induced Esophageal Dysmotility. An Experimental Study in Ex-Vivoguinea-Pig Isolated Esophagi

Background/Aims: Esophageal motor abnormalities are frequently found in patients with gastroesophageal reflux disease. The effect of bile in esophageal dysmotility is probably secondary to mucosal signaling to the muscular layer and not a transmural process. This study aims to identify the mucosa-muscular signaling path by receptors blockage in an experimental study. Methods: Fifteenguinea pig esophagi were isolated and ex-vivo esophageal contractility was assessed with force transducers. The esophagi were incubated in 100 µM ursodeoxycholic acid for 1 hour and 5 sequential contractions induced by 40 mM KCl spaced by 5 minutes were measured. After 30 minutes, esophagi specimens were incubated in 3 different smooth-muscle contraction antagonists: atropine (1µM) in 5, suramin (1µM) in 5 and genistein (1µM) in 5. The same protocol for contractions was repeated. Values are expressed as mean ± standard deviation and encompass the mean of five stimuli. Experimental procedures were approved by the University Institutional Review Board. Results: Contraction amplitudes after bile incubation but before antagonist incubation were 1.6±0.6 g, 1.2±0.8 g, and 1.2±0.4 g for atropine, suramin and genistein, respectively. Mean contraction amplitudes after antagonists instillation were 1.2±0.6 g, 1.4±0.5 g, 0.9±0.2 g, respectively. There was no different in contraction amplitude before and after instillation of atropine (p=0.188), suramin (p=0.488) or genistein (p=0.079). Conclusion: Our results show that blockage of cholinergic (atropine), purinergic (suramin) or tyrosine kinase (genistein) paths do not change esophageal dysmotility induced by bile. Other molecular path may play the role in this scenario.

Modulation of Summer Squash Growth and Productivity Via Spiritual Blessings (Biofield) Energy Treatment (SBET)

Background The increasing demand for sustainable and eco-friendly agricultural practices has led to the exploration of non-traditional methods to enhance crop yield and resilience. Spiritual Blessings (Biofield) Energy Treatment (SBET), a form of consciousness-driven energy healing, is increasingly being investigated for its potential to modulate biological systems at the cellular and molecular levels without use of chemical additives Objective This study aimed to evaluate the impact of SBET on the growth characteristics and overall productivity of summer squash (Cucurbita pepo L.). Methods The study was conducted using a controlled experimental design, where seeds and plots were divided into two groups: control and treated. The treated group received a remote SBET by a recognized practitioner, while the control group remained untreated. Both groups were maintained under identical environmental conditions (soil, water). Parameters such as germination rate, plant height, leaf area index, and total fruit yield were monitored over a full growth cycle. Results Results showed that plant height, number of branches, and total number of leaves per plant were significantly improved by 35.14% (p ≤ 0.001), 41.64% (p = 0.011), and 49.01% (p = 0.029), respectively, in the treatment group compared to the control group. Additionally, fruit length and total fruit yield (tons per hectare) were significantly increased by 39.68% (p = 0.002) and 15.92%, respectively, in the treatment group compared to the control group. Conclusion Exposure of SBET significantly improved both vegetative and reproductive development, yielding substantial increases in plant height, branching, and leaf production.

Interactions Between Natural Nuclear Reactors and Microbial Evolutionary Processes

The impact of ionizing radiation on genetic change is well established, yet the extent to which naturally occurring radiation fields have influenced evolutionary trajectories remains incompletely understood. This study examined correlations between microbial evolution and the radiation and geochemical environments associated with natural fission reactors, with emphasis on the Oklo–Bangombé system in present-day Gabon, Africa. The current paper compares plausible doserate regimes adjacent to reactor zones with published observations of radiationinduced phenotypes, geneexpression changes, and repair strategies in model organisms and complex biotas. This study further considers indirect mechanisms (e.g., water radiolysis, redox restructuring, tracemetal mobilization) by which natural reactors could have modulated ecological selection pressures over long timescales. The synthesis supports the plausibility of three interacting pathways: (i) increased mutation supply under low, chronic dose rates; (ii) selection in oxidantrich, redoxstratified niches; and (iii) metabolic subsidies (e.g., H₂) from radiolysis that support chemotrophic guilds. Although temporal–spatial associations exist between reactor activity and biological innovations preserved in Paleoproterozoic strata of Gabon, current evidence remains correlational rather than demonstrably causal. The study further outlines testable predictions and experimental designs capable of discriminating among these mechanisms.

The Effectiveness of Cognitive-Analytic Therapy in Women Diagnosed with Breast Cancer and Post-Traumatic Stress Disorder

The present study examines the effectiveness of Cognitive-Analytic Therapy (CAT) in women diagnosed with breast cancer and Post-Traumatic Stress Disorder (PTSD) on reducing PTSD symptomatology and improving their mental health state (depression, self-esteem, post- traumatic growth, quality of life, therapeutic alliance). Additionally, the investigation includes the determination of the demographic, socio-economic and medical factors’ impact on mental health indicators in women with breast cancer and PTSD. The sample was 188 women with breast cancer and PTSD at the Chemotherapy Unit of ‘Agios Andreas’ General Hospital in Patras. The questionnaire data were analyzed using descriptive and inferential statistical analysis in order to determine any statistically significant correlations between the experimental and the control group and between psychological scales and the demographic and socio-economic factors. The findings confirm the effectiveness of CAT in women with breast cancer and PTSD in reducing PTSD and depressive symptoms, improving self-esteem and quality of life, achieving greater post-traumatic growth, and fostering a better therapeutic relationship with the therapist. The demographic, socio-economic and medical factors examined affected dissimilarly each psychological scale, as statistically significant associations were found with some scales but not with others.

Reproductive Ability of Currant–Gooseberry Hybrids After Cryopreservation of Pollen in Liquid Nitrogen

The present study of the reproductive ability of currant and gooseberry hybrids after long-term cryopreservation of pollen in liquid nitrogen is of enormous importance for organizing the conservation of the diversity of this crop. Four distant interspecific hybrids of currant and gooseberry served as objects of the study. The pollen fertilizing ability was studied by pollination of allotetraploid Jošta with pollen of tetraploid hybrids stored for a year at ultra-low temperatures (–196°C) and freshly collected pollen (control pollination). Berry set in 2022, when pollinated with cryopreserved pollen, averaged 56.4% and was higher than in the control pollination (38.2%). In 2023, the number of berries set on average for all cross combinations in the experiment was also quite high (59.6%), but lower than in the pollination control (81.4%), which can be explained by unfavorable weather conditions during pollen collection in the year of establishment (2022). The obtained data specify that pollen of the allotetraploids does not lose its high fertilizing ability during its long-term cryopreservation. Berry setting, average berry weight and seed production are quite high and comparable to pollination with freshly collected pollen (control pollination variant). Seed germination and values of morphometric parameters of seedlings obtained from pollination with cryopreserved and freshly collected pollen in most cases have close values. Cryopreservation of pollen of currant-gooseberry hybrids for a year and more allows to preserve pollen for successful pollination and berry formation and is a reliable way to preserve germplasm.

Efficacy of Neemraj and Beauvitech on Abundance and Distribution of Arthropod Pests in Soil and Hydroponically Grown Strawberry

Strawberry is a commercially important crop which has gained global significance in terms of production and consumption. The strawberry farmers have been faced with economic losses due to attack from arthropod and phytophagus pests. To overcome this challenge, management of pests is important with reduced impact on beneficial organisms and the environment. Use of non-chemical approaches as an alternative to insecticide driven management is highly gaining popularity. Identification of the non-chemical production for management of strawberry pest such as entomopathogenic fungi and botanicals is critical. The need for compatibility of entomopathogenic fungi in crop production techniques with other insecticides to enhance efficacy is required. The interaction between these control agents could be synergistic, additive or even antagonistic. Synergistic interactions usually lead to enhanced effectiveness of the microbial control agents while reducing the adverse effects of the pesticides in combination. In this review a description of the compatibility of entomopathogenic fungus Beauvaria bassiana and botanical neem in strawberry pest management is given. The experiment involved five treatments: Neemraj, Beauvitech, Neemraj and Beauvitech, Ampligo and control. These treatments were allocated in a completely Randomized design in two separate greenhouse setups one with soil and other with pumice. The treatments were replicated four times. The data was subjected to analysis of variance (ANOVA) using the general linear model (GLM) procedure of the statistical analysis system (SAS) program, version 9.1. Tukey’s (HSD) test at P≤ 0.05 was used to separate significant means. The combination of Neemraj and Beauvitech was most effective in the management of pests in both hydroponics and soil grown media. Pest populations were reduced significantly as follows; thrips, Frankliniella spp 2.1, red spider mites Tetranychus species 11.5, tarnished plant bug Lygus species 3.0, there was 0% cricket Gryllus species damage and 0 number strawberry weevil in hydroponics cultures while in soil culture pest mean populations were: thrips 5.6, spider mites Tetranychus species 18.2, strawberry weevil (Otiorhynchus species) 7.0, tarnished plant bug 3.0 and there was 13.8% cricket Gryllus species damage on strawberry plants.

Molecular Control of Human Embryonic Development: A Comprehensive Study

Human embryonic development is a highly coordinated and complex process that transforms a single fertilized cell into a fully formed human organism. This process is governed by intricate molecular mechanisms involving genetic regulation, signal transduction pathways, and intercellular communication. This study explores key molecular pathways controlling human embryonic development, focusing on the roles of morphogens, transcription factors, signaling molecules, and epigenetic modifications. By reviewing the most recent literature and experimental studies, we aim to highlight the molecular orchestration that directs cell fate decisions, tissue patterning, and organogenesis in humans.

Standardizing Use of Mepiquat Chloride for Higher Productivity of Densely Planted Bt Cotton Variety

Standardizing use of mepiquat chloride for higher productivity of densely planted Bt cotton variety” was carried out at the Regional Research Station, Abohar and Regional Research Station, Faridkot during the kharif season of 2021 on Btcotton variety PAU Bt3. The soil of experimental field was silty loam at Abohar and sandy loam at Faridkot. The experiment was laid out in a split plot design with four plant spacings (67.5×15 cm, 67.5×30 cm, 67.5×45 cm and 67.5×60 cm) as main plots and five Mepiquat chloride levels (MC (5%) @ 375 ml ha-1 at squaring, MC (5%) @ 375 ml ha-1 at flowering, MC (5%) @ 750 ml ha-1 at squaring, MC (5%) @ 750 ml ha-1 at flowering and after 15 days and Control (No MC)) as sub plots with three replications. Lowest plant density (67.5×60 cm) and MC (5%) @ 750 ml ha-1 at squaring resulted in significant reduction of plant height, LAI (Leaf Area Index) and dry matter as compared to control at both the locations. Higher boll retention and opened bolls were observed in 67.5×60 cm and MC (5%) @ 750 ml ha-1at flowering. There was no significant effect of both the treatments factors on node count, monopods and sympods. Highest seed cotton yield was obtained with 67.5 ×30 cm (at Abohar) and 67.5×15 cm (at Faridkot) although these two spacing levels were at par with each other. MC (5%) @ 750 ml ha-1 at flowering resulted in 22.9% and 21.3% increase in seed cotton yield over control at Abohar and Faridkot, respectively. Different plant spacings and mepiquat chloride applications showed a non-significant effect on different fibre quality parameters except halo length at Faridkot. At both the locations, highest B:C ratio was observed in 67.5×30 cm and MC @ 750 ml ha-1 at flowering.

Trading on Impulse: The Role of ADHD, Impulsivity, and Gender in Financial Risk and Investment Outcomes

Background Attention-deficit/hyperactivity disorder (ADHD) in adulthood is increasingly recognized not only as a psychiatric condition but also as a trait with behavioral and occupational implications—particularly in high-stakes, fast-paced financial environments. Traits such as impulsivity, sensation seeking, and altered reward sensitivity may influence decision-making among individuals engaged in stock trading or high-risk investment professions. Objective This systematic review and meta-analytic synthesis aims to investigate the relationship between ADHD, impulsivity, gender differences, and financial risk behavior, with a particular focus on decision-making outcomes in real or simulated trading contexts. Methods Seventeen peer-reviewed studies published between 2008 and 2025 were included. Studies employed behavioral experiments, fMRI paradigms, neurochemical analysis (e.g., glutamate levels), and ecological financial assessments to examine impulsive traits and investment behaviors among adults with and without ADHD. Both clinical samples and occupational cohorts (e.g., brokers, retail investors) were analyzed. The analysis followed PRISMA 2020 guidelines. Results ADHD symptoms—particularly impulsivity and reward hypersensitivity—were associated with increased delay discounting, higher risk-taking, and diminished cognitive control in financial tasks. Neuroimaging data revealed hypoactivation in prefrontal control regions and hyperactivation in reward-related circuits (e.g., ventral striatum). Gender-stratified analyses showed that males with ADHD displayed stronger preference for immediate rewards, higher portfolio turnover, and greater volatility. Preliminary evidence also suggests an overrepresentation of ADHD traits in high-frequency trading roles. Conclusion ADHD-related impulsivity significantly modulates financial risk behavior, particularly in high-stakes and fast-paced contexts such as trading. A convergence of behavioral, neurobiological, and ecological findings suggests that males with ADHD are disproportionately prone to rapid, high-risk financial decisions, whereas females may demonstrate greater regulatory control. These insights underscore the need for gender-sensitive interventions, occupational screening, and tailored psychoeducation. As financial environments become increasingly automated and fast-paced, understanding the neurocognitive vulnerabilities of individuals with ADHD may not only protect personal outcomes but also enhance systemic financial stability.

The Effects of A 7-Minute HIIT Workout on Stress and Burnout in Outpatient Physical Therapists

Physical therapists (PTs) experience stress and burnout due to high job and productivity demands. Exercise can assist in decreasing stress and burnout, but finding the time to exercise can be difficult. This study examined the effects of a 28-day, 7-minute, high intensity interval training (HIIT) workout on PT’s stress and burnout levels. In this quasi-experimental pretest-posttest design, the Perceived Stress Scale, Maslach Burnout Inventory, grip strength, Functional Reach Test, and VO2max measures were administered on day 1 and day 28. Between the data collection period, participants completed a daily, 7-minute HIIT workout. A series of paired sample t-tests were computed to compare the pre- and post-HIIT raw values. Significant improvements were found in all comparisons post-HIIT (p< .001). Therefore, it was concluded that 7-minutes of HIIT can assist PTs in reducing stress and burnout levels. Employers should consider implementing a workplace wellness program to benefit their employees.

Increased Reaction Vessel Surface Area Decreases the Overall Mortality Rate of Rana catesbeiana Larvae during Chemically Induced Metamorphosis

Stimulating precocious metamorphosis in anuran larvae is an important pedagogical tool for understanding vertebrate development. However, historically, artificially provoking metamorphosis by immersing tadpoles in exogenous inducing agents (e.g., thyroxine, and iodine) compromises the longevity of the experimental animals, resulting in up to 100% mortality within a week. In our undergraduate teaching lab, we house our experimental tadpoles in circular glass dishes having a surface area of 182 cm2. Over the past four academic years this lab was performed, we observed 100% mortality of experimental animals within 10, 12, or 15 days when treated with 10-5 M, 10-6 M, or 10-7 M thyroxine, respectively. Here, we investigated whether increasing the surface area to 413 cm2 using square glass dishes would reduce the mortality of the treated animals. Omnibus Kaplan-Meier survival analysis demonstrates a statistically significant decrease in mortality in tadpoles reared in the larger square dishes compared to those housed in the smaller round dishes (P < 0.05). However, increasing the surface area of our reaction vessels could not rescue survivability of those tadpoles immersed in thyroxine, but did increase survivability of control tadpoles maintained in pond water (P < 0.01), tadpoles subjected to iodine (P < 0.05) or treated with actinomycin D (P < 0.05). These data demonstrate that increasing available reaction vessel surface area reduces overall tadpole mortality during chemically modified metamorphosis in an undergraduate teaching lab setting.

Response of Sorghum Varieties to Organic and Inorganic Fertilizer Strategies in Sudan Savanna of Nigeria: Productivity, Nitrogen, and Water Use Efficiencies

Experiments were conducted to evaluate the response of different sorghum varieties to micro-dosing fertilization strategies on yield and yield traits, as well as the impact on nitrogen fertilizer and water use efficiency (NUE and WUE). In addition, the benefit-cost ratio of sorghum cultivation under different fertilization strategies in the Sudan savanna zone of Nigeria was analyzed. The experiment included eight fertilizer micro-application strategies as well as two control and three sorghum varieties. Our results showed that most agronomic indicators differed significantly between years, varieties, and fertilization strategies. However, the application of 100g hill-1 poultry manure plus 3g NPK hill-1 resulted in the highest average grain yield > 2000 kg ha-1 at both study sites (BUK and Minjibir). This means that the grain yield is 86% and 132% higher than the average grain yield with zero fertilization. There were extremely significant differences between NUE and WUE fertilization strategies and varieties at the two sites. At BUK and Minjibir, NPK applied with 3 g of hill-1 had the highest NUE with an average of 37.6 and 40 kg grain/kg N. Application of 100 g of poultry manure plus 3g of NPK hill-1 resulted in the highest average WUE of 6.1 and 5.6 kg grain/mm for BUK and Minjibir, respectively. BUK (3.2) and Minjibir (3.6) had the highest net income and benefit-to-cost ratios when applying 3 grams of NPK per hill. The adoption of micro-dosing fertilization strategies by smallholder farmers provides a good opportunity to prevent long-term soil fertility limitations and thereby increase sorghum productivity and farmer incomes by recommending multiple-choice fertilization strategies for improved sorghum varieties.

Sesame (Sesamum indicum L.) seeds and di ammonium phosphate (dap) potential for controlling Striga seed germination, sorghum growth and grain yield

Sorghum is important staple food for feeding humans and animals in sub-Saharan African country. Nowadays, with the advent of climate change couple to the population growth, the crop is faced to new challenges wish are gradually affecting the productivity. In Niger, a dry land country where farmers are growing sorghum at a small scale for family subsistence, the crop is confronted to biotic and abioticonstraints including nutrient deficiency in the soil. Among those constraint, Striga is the one that causes huge damage to sorghum cultivation through yield reduction. In addition, nutrient depletion in the soil are causing weed progresses in farmer’s field at a large scale. In fact, to overcome Striga problem several control methods have been successfully tested, but some of them are efficient but not accessible to farmers. It is important to control the weed by using effective and accessible way for smallholder farme in Niger. The main objective of this study was to assess the effectiveness of different doses of sesame (0, 0.5 and 1.5 g) and DAP (0, 2 and 5 g) on Striga impact and sorghum grain yield. Two (2) grammes micro dosing utilization of DAP shows good result in Striga plant emergency and distribution along sorghum field. Concerning the three doses of sesame seed involve in this experiment, the micro dosing two (1.5 g) positively affect Striga effect. The combination sesame seed and DAP can significantly reduce Striga impact on sorghum cultivation and increased sorghum grain yield.

Ontogenes in Drosophila Melanogaster and a Model of Speciation

A new type of mutations-dominant lethals with a facultative manifestation -were discovered in D. melanogaster in 2000. These mutations were named conditional mutations. Under restrictive genetic conditions, the mutations manifest themselves as dominant lethals, whereas dominant lethality disappears under permissive conditions, displaying a set of other manifestations. The genes responsible for the emergence of conditional mutations were named ontogenes. The experiments with mutations in ontogenes have revealed the following processes: (1) genome editing in germline cells; (2) induction of high mutagenesis rates in germline cells of the mutants for ontogenes; (3) zygotic selection; (4) isolation of mutants; and (5) alterations in the lethality of mutants with time. The specific features in the manifestation of ontogenes together with the listed processes formed the background for construction of the model of speciation named the regeneration model. The event of speciation is represented as the regeneration of the working state of a genetic system disturbed by the emergence of a mutation in an ontogene. According to the model, it is ontogenes that are in charge of speciation and, eventually, the structure of living matter in the form of individual species. The significance of Mendelian protein-coding genes and Darwinian selection of the fittest according to these genes are doubtless but not paramount.

Pain Perception Modulates Synaptic and Somatic Plasticity in the Dentate Gyrus of Rats

An experimental study examines how pain perception alters synaptic and somatic plasticity in the dentate gyrus. It summarizes methods, key electrophysiological findings, and implications for pain‑related cognitive effects.

Genotype x Environment Interaction and Yield Stability Analysis of Some Chickpea (Cicer arietinum L.) Genotypes across Different Environments in Sudan

Genotype by environment (GE) interaction is considered to be among the major factors limiting the efficiency of breeding programs.Five chickpea genotypes were evaluated to study their adaptability and stability in eight environments of Sudan. The experiments were carried out in randomized complete block design (RCBD) with four replications in four locations in over two years (2017/2018 and 2018/2019).Stability was estimated using additive main effects and multiplicative interaction (AMMI) model.A Combined analysis of variance showed significant effects of genotypes, locations, years and their interactions on seed yield. Mean seed yield ranged between 1.88 and 1.45 t ha-1 for the genotypes FLIP 08-59 C and FLIP 09-187 C, respectively. The genotype FLIP 08-59 C gave the highest average seed yield and out-yielded the two checks, Shiekh Mohamed and Burgeig by 9.5% and 10.1%, respectively. The newly introduced (FLIP 08-59 C) showed non- significant differences in earliness as compared to the other two standard checks resistant to fusarium wilt disease and with good seed weight. The statistical analysis showed that genotype (G), environment (E) and their interaction (GEI) had highly significant (P ≤ 0.001) effect on seed yield. It also revealed that environmental differences accounted for 90.2% of the total variation. The results of data analysis displayed that the percentages of seed yield by environment, genotype and genotype environment interaction were 90.2%, 3.6% and 6.3%, respectively. The IPCA 1 and IPCA 2 axes (Principal Components) were highly significant P ≤ 0.001). The model for seed chickpea yield stability (the Additive Main Effect and Multiplicative Interaction (AMMI) model) showed that the G1 (FLIP 08-59 C) was adapted, high yielding and considered as stable genotype for Gezira, River Nile and Northern States of Sudan. In addition, this genotype is early flowering with non-significant differences in earliness as compared to the two checks and with good seed weight. Therefore, the above mentioned genotype FLIP 08-59 C could be recommended as commercially stable and high yielding cultivar and/or incorporated as breeding stocks in any future breeding programs aiming to produce high yielding genotypes of chickpea.

Histomorphomertric Analysis Of Hormonal Contraceptive Pills On Anterior Pituitary Gland In Female Wister Rats

Millions of women use hormonal contraceptive pills around the world and though the physical effects are thoroughly described in the literature and clinical setting. The psychological effects have been largely ignored until recently. Recent studies have found that the use of hormonal contraceptives has an effect on women’s hormones and psychological well-being. The aim of this present research was to check the effect of hormonal contraceptive pills on anterior pituitary gland among female wister rat histomorphometrically. Thirty (30) female wister rats of 3 month old weighing 150 – 200g were used for the study. They were divided into three (3) groups of 10 rats each. Group one (1) received Microlut Levonorgestrel 30mg at a dose of 0.18g/kg while group two (2) received a combination of microgynon levonorgesrel 150mg and ehinylestadiol 130mg at a dose of o.18g/kg in 5 days cycle while group three (3) serve as control group. The experiment last for 72 days. Histomorphometrical analysis of anterior pituitary gland was carried out. The findings revealed that combined oral contraceptive pills adversely cause the decrease in the cells of the anterior pituitary more than the progestin only pills.

Evaluating St. Augustinegrass (Stenotaphrum Secundatum (Walt.) Kuntze) Cultivars to Reduced Light Environments

St. Augustinegrass (Stenotaphrum secundatum (Walt.) Kuntze) generally has poor cold tolerance yet good to excellent shade tolerance. As mostly hot summers follow cold winters in USDA Hardiness Zone 7, severely damaging tall fescue (Lolium arundinaceum (Schreb.) Darbysh.) and centipedegrass (Eremochloa ophiuroides (Munro) Hack.), a St. Augustinegrass cultivar cold tolerant enough to be grown for shady lawns would greatly benefit home owners, recreational sites, and sod growers in the “transition zone.” Eight St. Augustinegrass samples were selected, including industry standards ‘Raleigh’ and ‘Palmetto’, plus ‘Palisades’ zoysiagrass (Zoysia japonica Steud.) for further testing from an established germplasm collection of material collected from lawns grown in USDA Hardiness Zone 7. Overall, based on 8-week greenhouse studies, the experimental lines had similar shade tolerance compared to commercial standards ‘Raleigh’ ‘Palmetto’ and ‘Palisades’ zoysiagrass. Field studies may be warranted to validate greenhouse studies to help further evaluate shade tolerance of experimental and commercial lines. Information generated supports the use of certain St. Augustinegrass selection in a wider environmental conditions such as reduced light environments (RLE).

Evaluation of Growth and some Growth Analysis Components in Sugar beet Genotypes Grown under Low Nitrogen Fertilizer Levels in Khartoum State- Sudan

This investigation was carried out at the Demonstration Farm of the College of Agriculture- University of Bahri during 2018/2019 winter season to evaluate growth (morphological) and growth analysis (physiological) components in some sugar beet (Beta vulgaris L) genotypes under different nitrogen levels to know how well sugar beet plant performs during the growing season, Thus, to provide information to assist producers in identifying and introducing superior genotype and good management of nitrogen application in AlKadro area. The experiment was laid out in split plot design. The genotypes used were namely, Blaladi. Strube Sudan 01/14, Strube Sudan 02/14, Strube Sudan 04/14, Strube Sudan 05/14 and Strube Sudan 06/14, and the nitrogen levels were viz, 0, 80 and 120 kg urea per ha; applied twice (at the sowing and then 4 weeks after sowing). The evaluated components were; leaf number/plant, leaf area index (LAI), root length, root diameter, fresh and dry weight of foliage/plant, fresh and dry weight of root/plant; all determined at 5 terms. While Crop Growth Rates (CGR), Relative Growth Rate (RGR) and Net Assimilation Rate (NAR); determined at different periods of growth (intervals). The analysis of variance (ANOVA) revealed that at 4 weeks after sowing (WAS): leaf number (14.33- 17.03) , root length (19.05 – 21.75 cm), root diameter ( 7.93- 8.40 cm) foliage fresh ( 186.93 – 292.06 g) and dry ( 69.00 – 94.10 g) weight per plant, root fresh (72.66 – 108.88 g) and dry weight ( 12.54 – 22.08 g) per plant differed significantly (P≤ 0.05); at 7 and 10 WAS leaf number (22.39 -35.73 and 26.91 – 38.47, respectively), LAI ( 3.725 -5.645) , fresh and dry root weight per plant ( 586.78 – 913.81an 189.06 – 326.43 g, respectively) differed significantly; at 13 WAS: dry foliage weight ( 69.00 – 94.10 g), LAI ( 2.603 – 4.744), root diameter (10.09 – 11.92 cm) differed significantly; at 16 WAS only dry foliage (44.34 – 73.48 g) weight reflected significance. All other cases reflected insignificant differences among the evaluated genotypes. Moreover, all the studied components reflected insignificant differences among the nitrogen fertilizer levels and likewise genotype x nitrogen interaction (G x N) at the 5 sampled terms. Nevertheless, CGR, RGR and NAR displayed insignificant effect on the studied components in the evaluated periods.

Detection of Smuggled Genetically Modified Crops and Assessment of its Environmental Impact in the Ethio -Sudan Trans Boundary Area, North West Ethiopia

Background The genetic material of the genetically modified crop has been altered to develop the necessary insect resistance features by introducing genes from the Bt (Bacillus thuringiensis) bacterium. The objective of this study was to find smuggled GM Bt crops in the Metema farming area and examine its environmental effects. Method An experimental; Completely Randomized Design (CRD) was used to collect crop samples in the study area. The CTAB (Cetyltrimethyl ammonium bromide) technique was used to isolate DNA from all transported samples, and the purity was determined using a Nano Drop spectrophotometer. Conventional PCR with particular primers for different Bt gene events was used to detect the presence of genes. Furthermore, utilizing Bt cotton specific primer sets, the prevalence of GM cotton was measured, and amplified fragments were confirmed using agarose gel electrophoresis. Result The PCR results revealed that 15 (33.3 percent) of the samples were Bt cotton smuggled from Sudan. The PCR assay also revealed the presence of GM maize. Moreover, the effects of GM genes on the environment were studied in diseased samples, and no transgenes were found. Furthermore, domestic and indigenous crops were used to determine horizontal gene transfers of GM genes to other crops, and the transgene was not found in any of the samples analyzed. Conclusion: In the current study, 28 (13.4%) of the 209 (100%) total analyzed samples were GM crops which indicated the presence of unauthorized GM seeds in the study area. Environmental impact studies and horizontal gene transfer data similarly revealed that the Bt gene was not transferred to other crops and had no harmful environmental effects. For a better understanding of the Impact of imported unauthorized GM seeds, more additional detection of GM events should be done by expanding the sampling site and sample types.

Clustering objects for spatial data mining: a comparative study

Spatial data mining (SDM) is searching important relationships and characteristics that can clearly exist in spatial databases. This content aims to compare object clustering algorithms for spatial data mining, before identifying the most efficient algorithm. To this end, this paper compare k-means, Partionning Around Medoids (PAM) and Clustering Large Applications based on RANdomized Search (CLARANS) algorithms based on computing time. Experimental results indicate that, CLARANS is very efficient and effective.

Effect of Bio-Controlling Methods (Proplis and Bacteria) on the 3rd_larval Instar of Galleria mellonella (Lepidoptera: Pyralidae)

The greater wax moth (Galleria mellonella L.) is one of the most destructive insect pests that threaten apiculture. Laboratory experiments were conducted in the Research Laboratory, College of Agricultural Studies, Sudan University of Science and Technology during the period from the 2nd_ of April to 29th_ of October 2021 to evaluate the lethal effect of Bee glue (proplis) and Bacillus thuringiensis var. Kurstaki (Btk) on the G.mellonella. Five concentrations (5%, 7.5%, 10%, 12.5% and 15%) of proplis extract and (0.62,1.25,2.5,5 and 10 mg/ml) of Bt were used for each proplis extract and Bt in a Completely Randomized Design. Powder ethanol extract of proplis at 15% concentration caused 83.3% mortality after 96 hrs of application, where as 10mg//ml concentration of Bt gave 93.3% mortality after 72 hrs of exposure. The statistical analysis revealed significant differences between all treatments and control. Additionally, all treatments (proplis/ /Bt) increased the mortality %.  

A Review on Drug Design by the Application of Computer

The process of creating new pharmaceuticals is incredibly costly and time-consuming, and it dates back millions of years to the time when only herbal remedies were used. Furthermore, the solvation energies of the ligand and receptor site are crucial to this process because partial to complete dedication must take place before binding. The full form of CADD is computer-added drug design. To enhance the design and discovery of, CADD stands for computational methodologies and resources. Smaller numbers of chemicals are chosen from extensive compound libraries for experimental testing. There is less screening. Pharmacogenomics’ main benefit is the ability to develop medication based on the genomiy organization of each individual. The immense potential of enzymes as therapeutic targets is exemplified by under R's leadership, Merck's strategy. Both the two exemplary PP'S were the (APS, a class of proteins, are making progress it will blossoms in computational thermodynamics.3D-QSAR mode was developed last year for the follow-up forecast of action of chemicals in a molecular database or newly created target's spatial organization is known.

Effect of Set Size and Fertilizer Type on Off- Season Onion (Allium cepa. L) Production

Two off-season experiments were conducted during 2015/16 and 2017/18 at Shambat Research Station Farm, Khartoum, Sudan, to investigate the effects of large (2-3 cm) and small (1-2 cm) sets size of onion variety Baftaim (S) and two chemical fertilizer (Urea 240 kg/ha) and NPK(15:15:15) (120 kg/ha) in addition to organic fertilizer (Elkhaseeb 20 tons/ha) and their combinations (Urea+ Elkhaseeb and NPK+Elkhaseeb) on off-season production. The experimental units were in split plot design, with set size and fertilizers as main and sub plots, respectively, with three replications. The yield was evaluated as bulb weight and yield/ha, whereas, the quality was evaluated as marketable and percentage of double and bolted bulbs, TSS and dry matter. The results reflected no significant differences in bulb weight due to set size, fertilizer type and their interactions, however, the heaviest bulbs were obtained by Elkhaseeb + urea and its interaction with the large set size in both seasons. The small set size gave the highest yield, organic fertilizer (Elkhaseeb) alone or in combination with urea gave the highest yield, whereas, the lowest one was obtained with the combination of organic fertilizer with N P K. The bulb quality was also affected by set size and fertilizer type as the highest marketable yield was obtained by small set size and fertilizer combination (organic + urea) or urea alone. No significant effects on TSS and dry matter percentage due to set size, fertilizer type and their interactions were noticed. The highest marketable yield, TSS and dry matter were obtained by Elkhaseeb (organic fertilizer) with mineral fertilizers (urea or NPK) with the small set size rather than organic or mineral fertilizer alone. It could be concluded and recommended that onion set size of about 1-2cm diameter with an organic - mineral fertilizer combination could improve off-season onion yield and quality. However, further studies are required to find the proper fertilizers dose.

Effects of Seed bed, Cultivars and Seed rates on Yield and Quality of Onion Sets (Allium cepa L.)

Field experiments were conducted during three successive winter seasons 2010/11, 2011/12 and 2012 /13, at Shambat Research Station Farm on clay loam soil pH 7.8, to study the effects of seed bed, cultivars and seed rates and their interactions on onion set yield and quality. Two seed bed (flat and ridge), two cultivars Kamleen and Baftaim (S) and three seed rates 4, 8, 12 g / m² (40, 80, 120 kg / ha) were tested. The experimental design used was 2*2*3 Factorial in spilt-split plot design with four replications. Yield was evaluated as set yield t / ha, whereas, quality was evaluated as percentage of large (2.9-2.0 cm) and medium (1.9-1.0 cm) set size according to diameter and average of single set weight (g). Cultivar Baftaim (S) gave the highest set yield t/ha and average of single set weight in the three seasons. The seed rate of 4 g/m² (40 kg / ha) resulted in highest average of single set weight and the highest number percentage of large set size. Increasing the seed rate increased set number and quality; the seed rate 12 g/m² (120 kg/ha) gave the highest percentage of medium set size.

Histo-Morphological Effect of The Small, Large Intestines and Stomach of Animal Models Treated With Aqueous Extract of Abelmoschus Esculentus

This study investigates the effect of Aqueous extract of abelmoschus esculentus on the microanatomy of the small, large intestine and stomach and the body weight of Wister rats. Twenty-one adults male wistar rats weighing between 100-120 grams were assigned into three groups consisting of seven rats each; Group A (control), Group B (low dose), and Group C (high dose). The rats in the control group were fed with fed with feed and water only while the rats in groups B and C were treated with 0.1mg/kg body weight and 3.0mg/kg body weight of abelmoschus esculentus respectively for 14 days. At the end of administration, the final weights of all rats were recorded before sacrifice using cervical dislocation and the small, large intestine and the stomach were harvested, processed and stained using H&E stain. The results were revealed as significant (p<0.05) increased in the mean body weight compared with the weight in the control groups and experimental groups. The treated animal groups revealed increased cellularity, focal metaplasia of the mucosal cells with villous disruption in the small intestine and dysplasia of the mucosal with loss of epithelial shape in large intestine. The stomach histology showed gastric pits with goblet cells smooth muscles layer and surface epithelium in the control group. Sections from the low dose treated group showed deep epithelical gastric pit areas with marked depletion of pits and goblet cells while the high dose treated group revealed dysplasia of gastric pits, goblet cells and smooth muscles appear mildly eroded.

Marginal SARS-CoV-2 Spike Protein Increases Interferon and Balances Cytokine Gene Expression

Some evidence confirms the paradoxical beneficial role of harmful antigens when used in highly diluted forms. In this experiment, we observed cytokine gene expression changes in Gallus gallus embryo after challenge with Delta SARS-CoV-2 RBD spike protein antigen, from a concentration of 10 µg/mL to a series of highly diluted forms in ethanol, along with controls. We have also studied pre-and post- experimental combined sets of higher (10 µg/mL) and significantly lower antigen concentrations (attogram level). Attogram and zeptogram level concentrations of the antigen showed consistently remarkable up-regulation of INF-α among different cytokine gene expressions. INF-β gene expressions at the zeptogram level of the antigen showed consistent changes, although not so outstanding. The pre-experimental set having attogram level antigen administered first, followed by a 10µg/mL antigen challenge, showed excellent cytokine balance. Other experimental groups, including the control sets, showed variable results at different concentrations.

Efficacy of Carumcarvi L. (Caraway), Eculaptuscamaldulensisdehnh (Red Gum) Andnigella Sativa (Black Seed) Against Greater Wax Mothgalleria Mellonellal.Innaeus (Lepidoptera:Pyralidae)

The greater wax moth considered one of the most worldwide-spread pests of the bee wax. This Study was designed to investigate the insecticidal activity of Carumcarvi, Eculaptus camaldulensis and Nigella sativa against 3rd larval instar of G.mellonellathrough laboratory screening. Five concentrations (5%, 7.5%, 10%, 12.5% and 15%) of each plant extract were used in this experiment. Mortality (%) was recorded after 24, 48, 72 and 96 hrs post treatment. The results showed C. carvi, E. camaldulensis and N.sativahave insecticidal activity against 3rd_ instar larvae of G. mellonella. The highest concentration (15%) caused 90% ,86.7% and 80% larval mortality after 96 hrs for C. carvi,E. camaldulensis and N.sativarespectively. The statistical analysis revealed that there is a significant difference (p< .001) between all treatments and control. The results also clearly demonstrate that the C. carvi are significantly more toxic than E. camaldulensis and N. sativa, whereas the LC50 values were 5.4% forC. carvi,6.0% for E. camaldulensis and 6.2% for N. sativa.

Biological Effects of High Radiofrequency Radiation on Wistar Rats: A Literature Review

Aim and Objective Despite the growing concerns about the relationship between exposure to radiofrequency radiation (RFR) and detrimental health effects due to the changes in biological processes of experimental animals, there is still ongoing debate on the significance of these findings in causing significant public health problems with the growing advancement in internet technology. The aim of this study is to review existing literature on the effects of high RFR on wistar rats. Method A search was conducted on Google scholar and PubMed to identify relevant peer-reviewed articles to be included into the review. Studies eligible for inclusion included free full text articles on wistar rats exposed to ≥ 2.45GHz RFR conducted in the past 5 years. Studies included in this review were written or transcribed in English language. From 286 titles, 36 eligible studies were included in the review and assessed for quality using the Strengthening the Report of Observational Studies in Epidemiology – Veterinary Extension (STROBE-Vet) quality assessment tool. Results Studies included in this review generally had good quality (>60%) based on the STROBE-Vet assessment. This review identified numerous biological changes in wistar rats exposed to high RFR including variations in biochemical, cholinergic, genetic, histopathologic, psychological, optical, and dermatological parameters. In this review, studies identified variations in protein and liver enzymes while high RFR was found to induce oxidative stress and cellular damage of exposed wistar rats compared to the unexposed groups. This was seen in the changes in protein, lipids, enzymatic and non-enzymatic antioxidants. Studies also identified changes in expression of genes and neurotransmitters with imbalance in hormones. In addition, this review identified structural changes of cells, tissues and organs indicative of apoptosis, damage and death. Exposed rats were identified to have behavioral changes indicative of anxiety and memory decline while studies identified optical and dermatologic changes in exposed rats compared to the unexposed. Conclusion With numerous biological changes identified in wistar rats exposed to high RFR, there is an increasing risk of detrimental health events giving the advancement in internet technology and limited regulations to control exposures to RFR. Therefore, studies should be conducted to identify strategies to mitigate human exposure to RFR while policies are developed and enforced to protect human health.

Integrated Management of Sclerotinia Sclerotiorum, An Emerging Fungal Pathogen Causing White Mold Disease

Sclerotiniasclerotiorum, the causal agent for white mold (Sclerotinia stem rot), is a devastating fungal pathogen. Currently, Sclerotinia is most commonly managed using the chemical fungicide which can lead to Sclerotinia resistance development, impacting biodiversity and interfering with key ecosystem services. In this regards, field experiments were conducted during 2017-18 planting seasons to evaluate the efficacy of different components viz. sawdust burning, stable bleaching powder, fungal and bacterial bio-control agents, chemical fungicide Rovral 50 WP and integration of different components for the management white mold disease of bush bean, mustard and garden pea in three different locations viz. in the field of Plant Pathology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Regional Agricultural Research Station (RARS), Burirhat, Rangpur and RARS, Ishurdi, Pabna, respectively. The results showed that different treatments displayed varying levels of effectiveness against the disease. All the treatments gave satisfactory reduction of white mold disease development and increased plant growth as well as yield of bush bean, mustard and garden pea. Among the treatments, integration of saw dust burning + soil amendments with Trichoderma based bio-fungicide + bacillus based bio-control agents + application fungicide Rovral 50 WP is the best treatment which reduced 97.49%, 77.72%, 72.26% white mold disease incidence and 84.61%, 81.14%, 71.01% white mold disease severity of mustard, bush bean and garden pea, respectively and increasing plant growth parameter as well as 52.16%, 27.74%, 36.97% yield of mustard, bush bean and garden pea, respectively. Application of only fungicide Rovral 50 WP also better treatment in reduction of white mold disease incidence and disease severity and increasing plant growth parameter as well as increasing yield of mustard, bush bean and garden pea. Soil amendment with fungal or bacterial bio-control agents also gave satisfactory results in reduction of white mold disease incidence and disease severity and increasing plant growth parameter as well as increasing yield of mustard, bush bean and garden pea. It could be concluded from the obtained results that integration between bio-control agents as a soil treatment and foliar application chemical fungicide might be useful as a good tool for controlling white mold disease caused by S.sclerotiorum and obtained higher yield of bush bean, mustard and garden pea under field condition.

Beneficial Impacts of Solanum aethiopicum L. in Diabetes Control

Garden egg nutritional values are numerous. However, few nutritional and epidemiological data exist on the impacts of garden egg consumption on diabetes control. In this experimentally-control designed nutritional study, the effects of scarlet garden egg species (Solanum aethiopicum L.) on lipoglycemic profile, weight control and, pancreas histoarchitecture in diabetic male Wistar rats were examined. Twenty-One adult male Wistar rats inducted with diabetes were randomly categorized into three groups (n = 7, each): Diabetic control (DC); Diabetic treated with aqueous extract of garden egg (DEE) and Diabetic fed with garden egg-supplemented diet (DSE). Animals were fed for six weeks according to the experimental design. Glycemic status and body weights were assessed twice weekly while lipid analysis was conducted at the entry and 6th week of the study. Oral glucose tolerance (OGT) test was conducted. Gross analysis and tissue histology of the pancreas were assessed by Hematoxylin and Eosin (H&E) staining technique. Statistical analysis was done using analysis of variance, and the results were expressed as mean ± S.E.M. at P < 0.05. Garden egg reduced mean body weight gain (DSE: 14.53%; DEE: 10.58%; P value = 0.04) and decreased blood glucose concentrations (DEE: 37.33%; DSE: 18.68%; P = 0.03) with corresponding improved lipid profile, glycemic tolerance and control (DEE > DSE) and, preserved pancreas histoarchitecture in diabetic Wistar rats. Solanum aethiopicum (garden egg) consumption (as fresh fruit or supplemented diet) preserves pancreatic tissue histoarchitecture and improves lipoglycemic profile and weight control in diabetic Wistar rats.

Effect of Soil and Foliar Application of Plant Nutrients on Purple Blotch and Tip-Burn of Garlic

An experiment was conducted in the field of Plant Pathology Division, BARI, Joydebpur to evaluate the efficacy of available plant nutrient for the development of purple blotch disease and tip-burn of onion. Eight different treatments viz. Potassium (MP fertilizer), Phosphorus (TSP fertilizer), Boron (Boron fertilizer), Zinc (ZnSO4 fertilizer), Calcium (CaSO4 fertilizer), Copper (CuSO4), Silicon (silica gel) and Manganese (MnSO4 fertilizer) were tested against purple blotch disease and tip-burn of garlic. The present study revealed that soil and foliar application of plant nutrients viz. Potassium, Phosphorus, Boron, Zinc, Calcium, Copper, Silicon and Manganese gave appreciable reduction of purple blotch disease and tip-burn incidence and increased plant growth parameters such as shoot and root growth as well as yield of garlic. Among the nutrients Potassium, Phosphorus, Silicon, Zinc and Calcium were performed better for reducing purple blotch disease severity, tip-burn disease incidence and increasing plant growth as well as yield of garlic. Application of Boron and Manganese also performed better than control. So, soil and foliar application plant nutrients Potassium, Phosphorus, Silicon, Zinc, Boron and Manganese may be recommended for tip-burn and purple blotch disease management and for garlic production in Bangladesh.

Expression of Concern: Potential Risks and Unknown Effects of mRNA Vaccines on Population Health (6th Rev). Damages Are Being Materialized

Several mRNA vaccines are used on the population in the U.S. I started predicting the dangers of mRNA vaccines before March 2021 and update my findings periodically. My prior model study enabled me to identify many flaws in clinical trials, side-effect evaluation methods and mechanism studies, and I also considered consistent failure in predicting drug side effects in the past and systematic failure of FDA in keeping out dangerous drugs from market. I found that the risks of vaccination cannot be determined by experiments alone and must be determined by using a combination of methods. By studying mRNA expression dynamics and kinetics, I predict that vaccination with mRNA vaccines may increase cancer risks, multiple organ failure risks, earlier death risks, genome alteration speeds by one or more mechanisms, alter the normal selection process for viral evolution resulting in more virulent viruses, and aggravate chronic diseases or cause healed diseases to relapse. Two root problems are practical inability to control expression sites and severe adverse reactions from repeated vaccination. Based on mRNA bio-distribution, the mRNA mainly strikes the liver and other vital organs, and poses grave dangers to persons whose vascular functional reserves are relatively small, or whose vascular systems are temporarily burdened by other causes such as viral infections or life activities. If an mRNA vaccine is administered on a pregnant woman by second or booster shots, spike protein synthesis in fetus brain disrupts the highly regulated protein synthesis processes, resulting in potential brain damages. In less than a year, most of my early predicted damages are being materialized or are on the track to hit the population. In this update, I present a benefits-and-risks map to show how the number of deaths caused by mRNA vaccines is grossly underestimated and why claimed benefits like 95% effectiveness rate and 90% death rate reduction are meaningless and misleading.

RETRACTED: Development of Biorational Management Package of Root Knot Nematode Disease of Country Bean

This article has been retracted on 10 November 2022. VIEW THE RETRACTION NOTICE (https://doi.org/10.14302/issn.2639-3166.jar-25-5841) A field experiment was conducted with a view to test the efficacy of Tricho-composts and integration of Tricho-composts, poultry refuse (PR), neem oilcake (NOC), saw dust burning (SDB) with chemical nematicide Furadan 5G (Carbofuran) for controlling root knot nematode of country bean through which growers will be benefited. The root-knot nematode infested soils were treated with Tricho-compost-1 @ 2 kg/pit, Tricho-compost-2 @ 2 kg/pit, Tricho-compost-1 @ 2 kg/pit + Furadan 5 G @ 20 g/pit, Tricho-compost-2 @ 2 kg/pit + Furadan 5 G @ 20 g/pit, poultry refuse @ 5-6 kg/pit + Furadan 5 G @20 g/pit , neem oil cake @ 500 g/pit + Furadan 5 G @ 20 g/pit and saw dust burning + Furadan 5 G @ 20 g/pit. It was revealed that all the treatments performed well in reducing root-knot nematode infestation and increasing plant growth and yield country bean compared to farmers’ practices. Among the treatments, integration of poultry refuse with Furadan 5G or Trichoderma based bio-fungicides Tricho-composts with Furadan 5G or neem oil cake with Furadan 5G are the best treatments in reducing root-knot nematode infestation and increasing plant growth as well as yield of country bean. Soil treatment with only Tricho-composts or integration of saw dust burning with Furadan 5G also performed better in reduction of root knot nematode disease and increasing plant growth as well as yield of country bean.

Stem Cell Differentiation Stage Factors (SCDSFs) Taken from Zebrafish Embryo during Organogenesis and their Role as Epigenetics Regulators able to Reverse Neurosensory Hearing Loss

This translational paper discusses differentiation‑stage factors from zebrafish embryos as epigenetic regulators with potential to reverse neurosensory hearing loss. It outlines experimental evidence, delivery concepts, and research gaps.

Attenuation of Microcystins Using Electron Beams and Gamma Radiation: A Study with Environment-Bound Conditions

Microcystins (MCs) are toxins profusely synthesized by cyanobacteria, causing livestock poisonings and endangering human health. We design and execute an experiment to investigate the attenuation (degradation) of microcystins by exposing them to gamma radiation and electron beams at doses of 0 (control), 3, 5, 10 and 15 kGy. The experimental conditions simulate microcystin contamination of aquatic environments; we thus consider (1) microcystins inside whole cells and extracellular dissolved in water, simulated by non-sonicated and sonicated cells, respectively, and (2) two acute microcystin concentrations within water. Toxicity tests of Microcystis aeruginosa detected immobilization (i.e., paralysis) of Ceriodaphniasilvestrii exposed to aqueous crude extracts of irradiated and non-irradiated M. aeruginosa (NPLJ-4 strain) at concentrations of 45 and 90 mg.L-1 (mg dry weight of freeze-dried material), and the results were analyzed using the Trimmed Spearman-Karber statistical program to obtain 48-h EC50, the average effective concentration causing immobility in 50% of organisms after 48 hours. We conclude that electron beams are effective physical agents for toxin attenuation (degradation) and reach 100% effectiveness at 5 kGy and above; their efficiency is two orders of magnitude greater than that of gamma radiation. This new body of information contributes to (1) remediating environmental water sources; (2) designing water/wastewater treatment facilities; (3) combatting chronic microcystin environmental contamination; and (4) inspiring further studies to promote the use of biomonitors (e.g., Cladocerans) to detect and evaluate microalgae contamination.

From High-Risk Behaviors to Problem-Solving Strategies: Acceptance and Commitment Therapy Effects on Addiction Susceptible Adolescents in Cyberspace

This study aimed to evaluate acceptance and commitment therapy (ACT) effectiveness in reducing high-risk behaviors and elevating problem-solving strategies in adolescents with addiction susceptibility in cyberspace. This study is longitudinal with quantitative methods of data collection and analysis. The target community in this research was 60 female students randomly selected from a Persian high school in Iran. Participants were randomly divided and placed equally into the experimental and control groups. The participants’ entry criteria were gender, aged from 15 to 18 years, addiction susceptibility, and high-risk behaviors cut-off points. Eight training sessions of ACT were presented in cyberspace for the experimental group between the pre-test and post-test intervals. Data collection instruments were the Iranian youth risk-taking scale, problem-solving strategies, and Iranian adolescents’ addiction susceptibility questionnaires. Participants were followed up after two months. The results using multivariate analysis of among subjects ANOVA showed ACT significantly influenced high-risk behaviors and its’ sub-scales except for the violence (p < .001).Furthermore, ACT affected the problem-solving strategies and its’ sub-scales instead of control, creativity, and confidence (p < .001). According to the findings, ACT decreased high-risk behaviors such as drug abuse, smoking, and unprotected sexual behaviors. These changes might be due to decreased helplessness and avoidance as well as increased acceptance and tendency to solve problems. Instead, problem-solving strategies were improved through ACT cyber treatment.

What do we know about Eye and Facial Protectors : Review for COVID-19 Pandemic and Possible Future Epidemics

Personal Protective Equipment (PPE) is mandatory for everybody in a threatening state, they are meant to provide safety and prevent injuries especially in hazardous issues. Facial guards are mostly used for providing eyes and face from splash-kind of hazards. During the COVID-19 pandemic, their use also was recommended to longer the use of masks or respirators, due to their shortage and the excessive demand of their reuse. This review gathered the information on eye and face protectors from various manufacturer, international and local guidelines; also authors’ experiment during the COVID-19 pandemic in Iran.

Response of Sugar Beet (Beta vulgaris L.) Growth, Yield and Yield Components to Compost and Phosphorus Fertilizers

Sugar beet is one of the sugar crops which widely grown in different regions of the world due to its advantages over Sugarcane. Several studies were conducted in Sudan to assess its adaptation and economic value. However, the aim of this experiment was to study the effect of application of compost and different levels of phosphorus fertilizer and their combination on Sugar beet (Beta vulgaris) growth attributes, yield and yield components. The study was conducted during the winter season 2018 –2019 at the farm of the College of Agriculture, University of Bahri, Alkadaro-Khartoum State, Sudan. The experiment was arranged in Randomized Complete Block Design (RCBD) with three replications and six treatments, namely the Compost (5t./ha.), Phosphorous (P2O5) (88kg./ha.), P2O5 (176 kg./ha.), Compost (5t./ha.)+P2O5 (88kg./ha.), Compost (5t./ha.)+ P2O5 (176Kg./ha.) and the Control (C) respectively. All cultural practices were carried out timely according to the recommendations of the Agricultural Research Centre in Sudan. Then the data pertaining the following agronomic traits were recorded, the leaf number; leaf dry weight (g), leaf area index (LAI) (cm), root diameter (RD) (cm) and root fresh weight (RFW) (g). The results of statistical analysis revealed the application of compost in combination with phosphorus displayed significant increase at 5% level for the leaf number (22.75), leaf area index (5.23), leaf dry weight(36.78), root diameter(69.67) and root fresh weight (422.68), followed by the application of compost alone compared to the control and other treatments. The study concludes that the combination of compost and mineral fertilizer (P2O5) proved to increase all Sugar beet growth and yield parameters.

Adolescent Girls in Guatemala: Can an Empowerment Program Change Perceptions of Gender Equity, Aspirations, and Communication?

While Guatemala has made great strides towards gender equality, it remains a country with vast disparities. Gender sensitization and empowerment programs during adolescence when gender attitudes are formed have the potential to diminish gender inequities. The Girl Rising Pilot Program (GR) is a 24-week curriculum designed for adolescent girls living in the Sololá region where 95% of the total population is indigenous and Mayan. The present pilot study evaluated outcomes of the GR Guatemala pilot program. Specifically, the evaluation examined whether there were differences between baseline and follow-up on perceptions of gender equity, aspirations, and communication for adolescent girls who participated in the GR program. A quasi-experimental study design with a one group pre- and post-test was implemented. The pilot evaluation study included all 265 participants at baseline who participated in the GR program. after controlling for significant variables, it was found that the gender equitable attitudes score and communications scale still significantly increased between baseline and follow-up. The results of this evaluation prove that gender sensitization programs play a key role in forming and changing gender attitudes during adolescence, and have the potential to alter their short and long-term attitudes and beliefs.

Effect of Nitrogen Fertilizer and Plant Spacing on Vegetative Growth of Sugar Beet (Beta vulgaris)

Despite the existing several Sugar manufacturing companies in Sudan, there is an acute shortage in sugar supply, therefore the government imports Sugar to bridge the gap. One of the strategies to be followed is the introduction of Sugar beet (Beta vulgaris) crop, mainly for sugar production. This crop has several advantages over Sugarcane such as short duration, less water requirement, in addition to other uses like animal feed. Therefore it became necessary to have good understanding of agricultural operations, cultural practices and adaptation. However, the main objective of this study was to assess the effect of Nitrogen fertilizer and plant spacing on vegetative growth of Sugar beet. This study was conducted at the farm of the College of Agriculture, University of Bahri, Alkadro, Khartoum State-Sudan; during the season 2016/2017. The experiment was arranged in Split plot in Randomized Complete Block Design with six treatments and four replications. Two plant spacing (15 and 20 cm.) were used as main plot, referred as (S1, S2) along with three levels of Nitrogen fertilizer (40, 80 and 120 kg/ha.), as subplot; referred as (N1, N2 and N3) and the control (0). Data regarding leaf number, leaf area index (LAI), leaf dry weight (g) (LDW), root diameter (mm.) and root fresh weight were recorded and statistically analyzed. The results showed S2 (20 cm) increased all the studied plant characters, namely the leaf number (29.139), leaf area index by (7.54), leaf dry weight (g) to (89.870), root diameter (mm) (94,992), root fresh weight (g) (695.80) compared to S1(1015 cm). On the other hand; the application of N3 (120 kg/ha.) increased the lead number (30.956), leaf Area Index (8.841), Leaf dry weight (102.47), root diameter (97.955) and root fresh weight (851.77) compared to S2 and S1 as presented in (table 4, table 5 and table 6).

What do we know about Torso Protectors and Foot Wears – A Review for COVID-19 Pandemic and Possible Future Epidemics

Personal Protective types of Equipment (PPE) are needed for everybody who would be in danger of threatening state; the COVID-19 pandemic raised the need and global demand for PPE, one of the most used and required equipment are gowns, coveralls, and torso protectors; which their shortage of supply raised the cost globally too. This review article gathered the guidelines and recommendations from manufacturers, international organizations, and self-experiments of authors for these utilities. And, provides recommendations on the level of protection needed for each condition.

The Biology of Fall Army Worm (Spodopterafrugiperda. J. E. Smith) in Sudan

The Fall armyworm (Spodoptera frugiperda) is considered among the economic important pests in Sudan. Therefore, it became necessary to study and understand its biology and find out the appropriate control measure (s). To achieve the above objective, experiments based on Randomized Complete Block Design (RCBD) were carried out during the period from September 2018 to August 2019, where the Fall armyworm (FAW) was reared in the laboratory and fed on a nutrient médium composed of Corn leaves. The results displayed the ranges of eggs laid by a female was 890–1169. The egg incubation period ranged between 3-13 days. The larval duration ranged between (13-50) days and the pupal duration was between (7-20) days under a temperature of 21-300c and a Relative Humidity (RH) of 65 ± 5%. The longevity of the adults was 1-20 days, and the range of the full lifecycle was (24-100). However, six generations of FAW were obtained within one year. This study concludes that in Sudan FAW breeds continously throughtout the year and it recommends further studies on the biology and effective management of this invasive pest

Evaluation of Inflammatory Serum Cytokines after Treatment with the Consciousness Energy Healing Based Proprietary Test Formulation on Combination of Cecal Slurry, LPS and E. Coli Induced Systemic Inflammatory Response Syndrome (SIRS) in Sprague Dawley Rats

Sepsis is a systemic inflammatory response to a confirmed or suspected infection. The transition from sepsis to septic shock causes high rate of mortality. The aim of this experiment was to evaluate the anti-inflammatory potential of the Biofield Energy Treated (Blessed) Proprietary Test Formulation and Biofield Energy Healing (Blessing) Treatment per se to Sprague Dawley rats on Cecal Slurry, LPS, and E. coli-induced systemic inflammatory response syndrome (SIRS) model. In this experiment, various proinflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6, IL-10, IL-12, 1L-17, and interferon-γ (IFN-γ) were analysed using ELISA. A test formulation was formulated including minerals (magnesium, zinc, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, vitamin E, cyanocobalamin, and cholecalciferol), Panax ginseng extract, β-carotene, and cannabidiol isolate. The constituents of the test formulation were divided into two parts; one section was defined as the untreated test formulation, while the other portion of the test formulation and three group of animals received Biofield Energy Healing Treatment remotely for about 3 minutes by a renowned Biofield Energy Healer Mr. Mahendra Kumar Trivedi. The level of TNF-α was significantly reduced by 40.50%, 85.36% (p≤0.01), 50.66% (p≤0.01), 87.38% (p≤0.01), and 58.63% (p≤0.01) in G5 (Cecal Slurry, LPS, and E. coli + Biofield Energy Treated test formulation), G6 (Cecal Slurry, LPS, and E. coli + Biofield Energy Treatment per se to animals from day -15), G7 (Cecal Slurry, LPS, and E. coli + Biofield Energy Treated test formulation from day -15), G8 (Cecal Slurry, LPS, and E. coli + Biofield Energy Treatment per se + Biofield Energy Treated test formulation from day -15), and G9 (Cecal Slurry, LPS, and E. coli + Biofield Energy Treatment per se animals + untreated test formulation) groups, respectively as compared to the disease control (G2) group. Additionally, the level of IL-1β was decreased by 17.04%, 15.56%, and 12.59% in G6, G8, and G9 groups, respectively as compared to the untreated test formulation (G4) group. The level of IL-6 was significantly (p≤0.001) reduced by 36.18%, 50.24%, 43.25%, 52.69%, and 38.23% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. The level of IL-10 was altered by 70.53%, 49.25%, 60.18%, 41.54%, and 58.89% in G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. Moreover, the level of IL-12 was decreased by 30.24%, 31.67%, 29.82%, 45.77%, and 50.54% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2. The level of IL-17 was reduced by 48.75%, 59.61%, 59.28%, 62.49%, and 58.65% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2. IFN-γ expression was reduced by 49.56%, 24.09%, 23.7%, 56.98%, and 44.94% in G5, G6, G7, G8, and G9 groups, respectively than G2. Overall, the data suggested anti-inflammatory potentials of the Biofield Energy Treated test formulation and Biofield Energy Treatment per se along with preventive measure on the animal with respect to various inflammatory conditions that might be beneficial various types of systemic inflammatory disorders specially sepsis, trauma, septic shock or any types of injuries. Therefore, the results showed the significant slowdown the inflammation-related disease progression and its complications in preventive treatment groups viz. G6, G7, G8, and G9.

Evaluation of Antioxidative Potential of the Biofield Energy Treated Proprietary Test Formulation on L-NAME and High Fat Diet-Induced Cardiovascular Disorders in Sprague Dawley Rats

The aim of this experiment was to assess the antioxidative potential of the Biofield Energy Treated/Blessed Proprietary Test Formulation and Biofield Energy Treatment/Blessing per se to the animals on NG-nitro-L-arginine methyl ester hydrochloride (L-NAME) and high fat diet (HFD)-induced cardiovascular disorders in Sprague Dawley rats using various functional biomarkers. A test formulation was formulated including minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, vitamin B9, vitamin B12, and vitamin D3), cannabidiol (CBD) isolate, Panax ginseng extract, and β-carotene. The test formulation’s constituents were divided into two parts; one part was denoted as the untreated, while the other part and three group of animals received Biofield Energy Healing/Blessing Treatment remotely for about 3 minutes by a renowned spiritual leader, Mr. Mahendra Kumar Trivedi. The expression of superoxide dismutase (SOD) was elevated significantly by 198.46%, 208.73%, 191.73%, 211.75%, and 198.82% in the G5 (L-NAME + HFD + the Biofield Energy Treated test formulation), G6 (L-NAME + HFD + Biofield Energy Treatment per se to animals from day -15), G7 (L-NAME + HFD + the Biofield Energy Treated test formulation from day -15), G8 (L-NAME + HFD + Biofield Energy Treatment per se plus the Biofield Energy Treated test formulation from day -15), and G9 (L-NAME + HFD + Biofield Energy Treatment per se animals plus the untreated test formulation) groups, respectively than disease control group (G2). Moreover, the level of glutathione peroxidase (GPx) was significantly increased by 61.94%, 118.49%, 82.96%, 141.89%, and 262.02% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. Lipid peroxidase (LPO) was decreased by 14.21%, 30.98%, 38.66%, and 32.67% in the G6, G7, G8, and G9 groups, respectively than G2 group. Additionally, the level of myeloperoxidase (MPO) was decreased by 28.46%, 10.87%, 12.41%, and 13.35% in the G6, G7, G8, and G9 groups, respectively than G2. Further, the level of oxidized low density lipoprotein (LDL) was reduced by 65.38%, 65.11%, 71.53%, 79.26%, and 66.57% in the G5, G6, G7, G8, and G9 groups, respectively than G2. Besides, in heart tissues, the level of catalase (CAT) was significantly increased by 68.20%, 63.69%, 126.03%, 124.54%, and 112.23% in G5, G6, G7, G8, and G9 groups, respectively than G2 group. Moreover, in kidney tissues, the level of CAT was significantly increased by 22.48%, 23.43%, and 10.95% in the G6, G7, and G9 groups, respectively than G2. Overall, the data suggested a significant antioxidant activity by increasing the levels of SOD, CAT, GPx, and reducing the levels of LPO, MPO, and oxidized-LDL in various tissue fluids and that might be beneficial for cardiovascular disorders. Therefore, the study outcomes showed the significant slowdown the oxidative stress-related cardiovascular disease progression and its complications in the preventive treatment groups viz. G6, G7, G8, and G9.

Evaluation of Antioxidative Potential of the Biofield Energy Treated Proprietary Test Formulation on L-NAME and High Fat Diet-Induced Cardiovascular Disorders in Sprague Dawley Rats

The aim of this experiment was to assess the antioxidative potential of the Biofield Energy Treated/Blessed Proprietary Test Formulation and Biofield Energy Treatment/Blessing per se to the animals on NG-nitro-L-arginine methyl ester hydrochloride (L-NAME) and high fat diet (HFD)-induced cardiovascular disorders in Sprague Dawley rats using various functional biomarkers. A test formulation was formulated including minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, vitamin B9, vitamin B12, and vitamin D3), cannabidiol (CBD) isolate, Panax ginseng extract, and β-carotene. The test formulation’s constituents were divided into two parts; one part was denoted as the untreated, while the other part and three group of animals received Biofield Energy Healing/Blessing Treatment remotely for about 3 minutes by a renowned spiritual leader, Mr. Mahendra Kumar Trivedi. The expression of superoxide dismutase (SOD) was elevated significantly by 198.46%, 208.73%, 191.73%, 211.75%, and 198.82% in the G5 (L-NAME + HFD + the Biofield Energy Treated test formulation), G6 (L-NAME + HFD + Biofield Energy Treatment per se to animals from day -15), G7 (L-NAME + HFD + the Biofield Energy Treated test formulation from day -15), G8 (L-NAME + HFD + Biofield Energy Treatment per se plus the Biofield Energy Treated test formulation from day -15), and G9 (L-NAME + HFD + Biofield Energy Treatment per se animals plus the untreated test formulation) groups, respectively than disease control group (G2). Moreover, the level of glutathione peroxidase (GPx) was significantly increased by 61.94%, 118.49%, 82.96%, 141.89%, and 262.02% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. Lipid peroxidase (LPO) was decreased by 14.21%, 30.98%, 38.66%, and 32.67% in the G6, G7, G8, and G9 groups, respectively than G2 group. Additionally, the level of myeloperoxidase (MPO) was decreased by 28.46%, 10.87%, 12.41%, and 13.35% in the G6, G7, G8, and G9 groups, respectively than G2. Further, the level of oxidized low density lipoprotein (LDL) was reduced by 65.38%, 65.11%, 71.53%, 79.26%, and 66.57% in the G5, G6, G7, G8, and G9 groups, respectively than G2. Besides, in heart tissues, the level of catalase (CAT) was significantly increased by 68.20%, 63.69%, 126.03%, 124.54%, and 112.23% in G5, G6, G7, G8, and G9 groups, respectively than G2 group. Moreover, in kidney tissues, the level of CAT was significantly increased by 22.48%, 23.43%, and 10.95% in the G6, G7, and G9 groups, respectively than G2. Overall, the data suggested a significant antioxidant activity by increasing the levels of SOD, CAT, GPx, and reducing the levels of LPO, MPO, and oxidized-LDL in various tissue fluids and that might be beneficial for cardiovascular disorders. Therefore, the study outcomes showed the significant slowdown the oxidative stress-related cardiovascular disease progression and its complications in the preventive treatment groups viz. G6, G7, G8, and G9.

Evaluation of Anthelmintic Activities of Fractions of Acanthus Montanus (Acanthaceae) on Adult Heligmosomoides Bakeri (Nematoda, Heligmosomatidae)

Acanthus montanusNees T. Anderson (Acanthaceae) has been employed in folk medicine for treatment of different kinds of ailment, but there is dearth of documented information on its therapeutic activities against parasites. In this study, pulverized Acanthus montanusleaf was subjected to four different extraction techniques. The percentage of yields were 25.58%, 31.42%, 11.58% and 3.00% weight by weight (w/w) of crude ethanol extract (CEE), aqueous (AQ), n-butanol (BUT) and chloroform (CHLO) portions, respectively. All the extracts, excluding the chloroform portion were administered to worm-infested mice per os at dose of 1.2 g/kg, 1.4 g/kg, 1.7 g/kg and 2.0 g/kg each for five days consecutive. Mice were euthanized and the adult worm counted for rates of deparasitization. The aqueous extract did not cause significant deparasitization even at the highest dose of 2.0 g/kg. The CEE caused significant (p<0.05) deparasitization rate of 72.35% at 2.0 g/kg dose. The n-butanol portion caused significant (p<0.05) deparasitization rates at doses between 1.4 mg/kg and 2.0 mg/kg (86.17% and 97.04% respectively) compared to figures from distilled water-treated mice (Control) as well as those from mice treated with the aqueous or crude ethanol portion. The 97.04% deparasitization produced by the 2.0 g/kg dose was not stastistically different (p>0.05) from the 100% deparasitization obtained using albendazole at the manufacturer’s recommended dose of 10 mg/kg. This study has demonstrated that the n-butanol extract of Acanthus montanus leaf has profound anthelmintic activity against experimental Heligmosomoidesbakeri infection in mice. Further phytochemical analysis and evaluation is being advocated in large animals and possibly human subjects.  

Evaluation of Immunomodulatory Effect of a Novel Test Formulation in D-Galactose-Induced Aging Dysfunction in Sprague Dawley Rats

The aim of the study was to evaluate the immunomodulatory activity of the Biofield Treated/Blessed proprietary test formulation consisting of essential ingredients viz. minerals (zinc, magnesium, iron, and copper) and vitamins (B6, B12, and D3) in male Sprague Dawley rats. Each ingredient of the test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Healing Treatment/Blessing, while the other part was defined as the Biofield Energy Treated/Blessed sample, which received the Biofield Energy Healing Treatment/Blessing by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi remotely. Additionally, three group of animals were also received Biofield Energy Treatment per se (at day -15) under similar conditions. The parameters were assessed such as immune biomarkers (IgM, IgG, IgA, IgE, CD4+, CD8+, and CD28+), biochemistry and hematology and histopathology. The experimental results showed IgG level was significantly increased by 10.70% and 8.03% in the G6 (Biofield Energy Treatment per se at day -15) and G8 (Biofield Treatment per se to animals plus Biofield Treated test formulation from day -15) groups, respectively as compared with untreated test formulation (G4). Additionally, CD8+ count was significantly increased by 20.67% in the G8 group, while CD28+ count was significantly increased by 11.70%, 8.32%, and 9.82% in the G7 (Biofield Energy Treated test formulation at day -15), G8, and G9 (Biofield Treatment per se (day -15) to animals plus untreated test formulation) groups, respectively after Biofield Energy Treatment to the animals as compared with the untreated test formulation. In hematological analysis, platelet count was increased in the G5, G6, G7, G8, and G9 groups by 40.69%, 27.95%, 26.67%, 38.58%, and 28.28%, respectively compared with the disease control (G2) group. Biochemical parameters showed significant decrease in the level of creatinine by 32.14% in the G9 group as compared with the G2 group. Further, animal body weight, feed intake, relative organ weight, and histopathological findings of all the tested groups did not show any abnormal findings with respect to the safe and non-toxic treatment strategies. Overall, the experimental data concluded that the Biofield Energy Treated/Blessed test formulation showed considerable improved cellular and humoral immune response as compared with the untreated test formulation. Thus, the Trivedi Effect®-Biofield Energy Healing Treatment per se and the test formulation has the significant capacity for immunomodulatory effect, stress management and anti-aging by improving overall health.  

The Efficacy of Some Plants Extracts on Fallarmyworm (Spodopterafrugiperda, J.E. Smizh) in Sudan

The Fall armyworm worm (FAW) (Spodopterafrugiperda) is one of the important economic pests which goes on several field crops and causes serious damage. The aim of this study was to look for efficient, less cost and environmentally friend plant extract for controlling Fall armyworm worm in cereal crops. A Complete Randomized Block Design (CRBD) experiments with three replications were conducted in the laboratory to investigate the insecticidal effects of four plant extracts consisting of ethanolic extract of Neem (Azadirachta indica) seeds, Black pepper (Piper nigrum) seeds, Usher (Calotropis procera ) leaves and water extracts of Argel (Solenostemma argel) leaves on larvae of the Fall armyworm (FAW) (Spodoptera frugiperda). Newly emerged larvae of FAW were treated topically by 4 concentrations (10, 25, 50 and 75%) of each extract, and then the larval mortalities were calculated after 24, 48 and72 hrs. The results showed the highest concentrations (75%) of the three ethanolic extracts gave higher mortality percentages (100%) after 72 hrs of exposure, compared with other concentrations. Also, these were not significantly different from the recommended dose of the standard pesticide “Spinosad”. On the other hand, Argel water extract showed no effect on the (FAW) larvae. It is recommended that this experiment to be replicated under different environments.

Evaluation of Renal and Cardioprotective Potential of the Biofield Energy Treated Proprietary Test Formulation on L-NAME and High Fat Diet-Induced Cardiovascular Disorders in Sprague Dawley Rats

The aim of this study was to evaluate the impact of Biofield Energy Treated/Blessed Proprietary Test Formulation and Biofield Energy Treatment/Blessing per se on kidney biomarkers on L-NAME and high fat diet (HFD)-induced cardiovascular disorders in Sprague Dawley rats. In this experiment, the functional kidney biomarkers such as epinephrine/adrenaline, inducible nitric oxide synthase (iNOS), angiotensin-II, C-reactive protein (CRP), and renin were measured using ELISA assay. A test formulation was formulated including minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (vitamin C, vitamin B6, vitamin B12, vitamin B9, and vitamin D3), cannabidiol (CBD) isolate, Panax ginseng extract, and β-carotene. The components of the test item were divided into two; one section was defined as the untreated test formulation, while the other part and three group of animals received Mr. Mahendra Kumar Trivedi’s Biofield Energy healing/Blessing remotely for about 3 minutes. The results showed that the level of adrenaline was reduced by 31.62%, 19.58%, 34.32%, 37.07%, and 29.87% in the G5 (L-NAME + HFD + the Biofield Energy Treated test formulation), G6 (L-NAME + HFD + Biofield Energy Treatment per se to animals from day -15), G7 (L-NAME + HFD + the Biofield Energy Treated test formulation from day-15), and G8 (L-NAME + HFD + Biofield Energy Treatment per se plus the Biofield Energy Treated test formulation from day-15), and G9 (L-NAME + HFD + Biofield Energy Treatment per se animals plus the untreated test formulation) groups, respectively as compared to the disease control group (G2). Moreover, the level of iNOS was reduced by 56.76%, 49.51%, 61.79%, 57.63%, and 62.44% in the G5, G6, G7, G8, and G9 groups, respectively, as compared to the disease control group (G2). Additionally, the level of angiotensin-II was decreased by 41.09%, 34.92%, 60.65%, 53.28%, and 60.09% in the G5, G6, G7, G8, and G9 groups, respectively, as compared to the G2 group. The level of CRP was decreased by 47.21%, 38.89%, 59.81%, 55.52%, and 64.02% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. Besides, the level of renin was decreased by 20.27%, 20.13%, 12.99%, and 25.73% in the G5, G7, G8, and G9 groups, respectively as compared to the G2 group. Overall, the data suggested significance improvement of vital functional kidney biomarkers of the Biofield Energy Treated/Blessed test formulation and Biofield Energy Treatment per se along with preventive measure on the animal with respect to various pathological conditions that might be beneficial various types of cardiovascular disorders. Therefore, the results showed the significant slowdown the inflammation-related cardiovascular disease progression and its complications/symptoms in the preventive Biofield Energy Treatment group per se and/or Biofield Energy Treated/Blessed Test formulation groups (viz. G6, G7, G8, and G9).

Evolutionary Conservation of Hox Genes in Vertebrate Brain Development

Hox genes, their conserved derivatives, and the pathways responsible for their expression have been extensively studied in the fruit fly, Drosophila melanogaster;the experimentation done in the Drosophila model system has given developmental biologists tools to better understand the role and significance of Hox genes and their derivatives in anterior-posterior axis determination in the Drosophila embryo. Along with this, Drosophila research opened up the door to investigation on the conservation of Hox genes between vertebrates and invertebrates. Comparative embryology in mice, chickens, pufferfish, and zebrafish have shown conserved Hox gene expression patterns specifically along the anterior-posterior axis. Recently, comparative analysis performed on dorsal-ventral axis formation showed that patterning and segmentation of the spinal cord is influenced by the action of Hox genes as well. This review will briefly consider the evolution of the vertebrate brain and the evolution and conservation of Hox genes in regulating hindbrain patterning and spinal cord development.

Insecticidal Activity of Cyperus rotundus L. and Datura stramonium L. Co-Administered with Sesame Oil Against African Bollworm Helicoverpa armigera Hübner (Lepidoptera: Noctuidae)

African bollwormis a worldwide-spread species that shows a high degree of polyphagia and it is considered as one of the main agricultural pests in the world. Laboratory experiments were conducted in the Research Laboratory, College of Agricultural Studies, Sudan University of Science and Technology to evaluate the lethal effect of C. rotundusand D. stramonium on the H. armigeraand to investigate the synergistic effect of sesame oil and tested extracts by dipping methods. Five concentrations (4%, 6%, 8%, 10% and 12%) were used for each plant extract in a Completely Randomized Design. Tubers ethanolic extract of C. rotundus at 12% concentration caused 90% mortality after 72 hrs of application, whereas 12% concentration of seeds ethanolic extract of D. stramonium generate only 70% mortality after 72 hrs of exposure. When sesame oil was added to each concentration of ethanolic extract of C. rotundus and D. stramonium it exhibited a synergistic effect. In fact, the 10% concentration scored 53.3% and 76.7% mortality, however, when mixed with sesame oil the mortality increased significantly to 83.3% and 100% for D. stramonium and C. rotundus respectively after 72 hrs of exposure. This  study clearly demonstrates that both tested plants have a lethal effect on the larvae of the African bollworm. However, tubers ethanolic extract of C. rotundus seems to be significantly more toxic than the seeds ethanolic extract of D. stramonium. This study also revealed that sesame oil has a synergistic effect when added to these plant extracts.  

Efficacy of Ricinus communis L., Cassia occidentalis L. and Bacillus thuringiensis against Helicoverpa armigera Hübner (Lepidoptera: Noctuidae)

Helicoverpaarmigera is one of the most destructive pests of field crops worldwide. The Study was designed to investigate through laboratory screening the insecticidal activity of Ricinus communis, Cassia occidentalis and Bacillus thuringiensis var. Kurstaki (Btk) and their combinations against 2nd larval instar of H. armigera. Five concentrations (4%, 6%, 8%, 10% and 12%) of each plant extract and (0.62, 1.25, 2.5, 5, and 10 mg/ml) of Bt were used in this experiment. Mortality (%) was recorded after 24,48,72 and 96 hrs post treatment. The results showed that R. communis, C. occidentalis and Btkhave insecticidal activity against 2nd instar larvae of H. armigera. The highest concentration (12%) caused 80% and 70% larval mortality after 96 hrs for R. communis and C. occidental respectively. The statistical analysis revealed that there is a significant differences between all treatments and control. Additionally, all combinations (plant/plant or plant/Bt) increased the mortality %. The results also clearly demonstrate that the R. communis are significantly more toxic than C. occidental where the LC50 values were 6.4 % for R. communis and 8.1% for C. occidental. On the other hand LC50 value for Bt was 0.41 mg/ml. The binary mixture of R. communis and C. occidental have a potentiation effect after 48 hrs. Regarding the mixture of Bt and plant extracts the results revealed that R. communis and Bt mixture induced a potentiation effect whereas C. occidental and Bt mixture induced a an additive effects.  

Factors and Conditions that Influence Teenage Pregnancy among In-school Adolescents in Umuahia North Local Government Area (L.G.A) of Abia State, Nigeria

Introduction Adolescence is a critical stage in human development that is characterized by peer pressure, confusion, exuberance and experimentation, particularly with sexual relationships. This is why attention should be paid to adolescents’ reproductive health issues so as to reduce their exposure to aggressive sexual activities which could expose them to sexually transmitted diseases, unwanted pregnancies and others. This study aimed at reducing the factors and conditions that influence teenage pregnancy among in-school adolescents in Umuahia North LGA of Abia State. Materials and Methods The study used a cross sectional descriptive study. A randomly selected sample of 416 adolescents between the ages of 13-19 years were studied. Structured self-administered questionnaire was used for data collection. Descriptive statistics, using frequencies, percentages and means were utilized for data analysis. Results The study found that 198 (47.6%) of the respondents were sexually active and that 89 (45%) of them had been pregnant. More than half 103 (52%) of the study group indicated that peer pressure influenced their sexual activities. About 46(51.7%) of the adolescents said they were pregnant so as to keep the new born baby with motherless babies homes. Conclusion Therefore, there is need for increased sex education for in-school adolescents so as to highlight the effects of teenage pregnancy on adolescents.

Analgesic and Anti-Pyretic Activities of the Root Bark of Rutidea Parviflora (Rubiaceae)

This study aims at investigating the antipyretic activity of different solvent fractions of the root bark of Rutideaparviflora(Rubiaceae). This plant is used ethno-botanically by the people of Ethiope East-West Local Government Area of Delta State, Nigeria to treat various ailments such as inflammation, fever and pain. This necessitated this research to validate its local use, due to the scanty literature and information present about this plant. It has also shown some anti-cancer and anti-inflammatory activity in previous researches. The present study is a randomized control study. Acetic acid induced writhing was employed for analgesic testing. Acetic acid was used to induce writhing in Wistar rats which were divided into fourteen (14) groups. The groups were administered extracts and fractions of the plant (200 mg/kg and 400 mg/kg). The animals were observed for number of writing movements and the percentage writhing was calculated. Baker’s yeast induced pyrexia was employed for the antipyretic testing. The animal groups were administered extracts and fractions of the plant (200 mg/kg and 400 mg/kg), with Paracetamol as the standard drug (100 mg/kg) and Normal saline (control) for both experiments. The body temperature of the rats was measured rectally over a period of five (5) hours. All values of P<0.05 were taken as significant. The organic extract, aqueous extract and various fractions (n-hexane, ethyl-acetate, n-butanol and aqueous) produced significant inhibition of writhing responses and pyrexia in a dose dependent manner and time dependent manner respectively. The aqueous extract at a dose of 400mg/kg showed the greatest reduction in writhing, 91.58% compared to the standard drug (paracetamol) which may suggest that the fraction possesses better efficacy than paracetamol as an analgesic. The observed activities could be attributed to these bioactive compounds: Palmatine, Urs-12-ene-24-oic-3-oxo-methyl ester and Gallic acid contained in R. parviflora.

Connecting the use of Biofertilizers on Maize silage or Meadows with Progress in Milk Quality and Economy

A systematic use of biofertilizers can improve both the quality of a farming system and the parameters of milk. Some issues related to biofertilization experiments on six farms in the Po Valley (NW Italy) involved in the production of milk from dairy cattle fed maize silage or grazed on hay produced from permanent meadows are reported in this paper. Biofertilized maize was found to lower the live stem pH by about 2.3%, and NIR spectroscopy foreshadowing major changes in the composition. Overall, the plant silage was improved in quantity (+10%) but also in quality, as shown by the delayed maturity stage of the leaves (crop maturity index -4%), the lower indigestible NDF content (-7%), and the higher digestible carbohydrates and protein in the whole plants. Such favorable feeding conditions, together with the improved palatability of the feed ration, boosted the nutrient values of the protein (+4.6%) and fat contents (+5.7%) in the milk. Moreover, the functional properties of the milk were ameliorated, as testified by the higher levels of vitamin A (+27%) and vitamin E (+25%) and the reduced levels of saturated fatty acids (-6%), especially myristic (-18%) and stearic (-32%) acids, while the unsaturated acids increased by 15%. As far as economy aspects are concerned, the biofertilization of maize for silage has led to consistent rewards pertaining to the marginal price of the milk, which in turn has led to a value chain increase of about 9%, because of the fields cultivation, but mainly of the cow transformation in milk quality issues. On another farm, intensive maize was substituted with permanent biofertilized meadows, over a greening path, and a + 17% value chain increase was obtained that already derived mainly from the best price for milk quality parameters. Such an evolutionary leap toward a new vision of sustainable agriculture for the environment and for animals, in which a better quality of products, animal welfare and company budget are combined with soil biofertilization, can be considered a bonanza.

Communicating Branch of the Mental Nerve and Facial Nerve

As peripheral branches of the mandibular nerve, the mental nerve and facial nerve communicate with each other. However, investigations have not always been described in the classic anatomical texts. It remains unknown how nerve fibers of this communicating branch converge at the micro level. Thus, the objective of the present study was to observe in detail the macro and micro levels of the communicating branch of mental and facial nerves. We used five cadavers (10 samples) to conduct experiments in anatomical practice at Tokyo Dental College. A macroscopic observation was made, and the communicating branch of the mental and facial nerves was removed as a single mass. We created serial sections of this branch using the standard method and observed communicating branches of these two nerves under microscopy. As a result, the communicating branch of the mental and facial nerves was completely fused at the perineurium level. It has been reported that the mental nerve includes a small amount of autonomic nerve fiber. As for these findings, similar findings were observed for all 5 bodies and 10 sides. Thus, we believe that autonomic nerve fibers derived from the facial nerve converge with the mental nerve via this communicating branch.

Mining Frequent Sequential Patterns

In recent times, the urge to collect data and analyze it has grown. Time stamping a data set is an important part of the analysis and data mining as it can give information that is more useful. Different mining techniques have been designed for mining time-series data, sequential patterns for example seeks relationships between occurrences of sequential events and finds if there exist any specific order of the occurrences. Many Algorithms has been proposed to study this data type based on the apriori approach. In this paper we compare two basic sequential algorithms which are General Sequential algorithm (GSP) and Sequential PAttern Discovery using Equivalence classes (SPADE). These two algorithms are based on the Apriori algorithms. Experimental results have shown that SPADE consumes less time than GSP algorithm.

Effect of Hyamine-1622 Cationic Surfactant on Pertraction of Cerium (IV) Cations Through Emulsion Liquid Membranes

The present study aims to shed light directly towards the extraction of (IV) cerium ions using "liquid surfactant membrane" technology, "LSM" developed in the presence of synergistic cationic and nonionic materials. The effect of various factors such as Ce (IV) transport, synergistic surfactants, curing ratio, stir speed, temperature, and mixing time between the carrier and the cerium ion on the extraction rate was studied by LSM taking into account surfactant agents. The positive effect of benzethonium chloride "Hy-1622" on the extraction of cerium ion was demonstrated by LSMs technique. Experiments confirmed the efficiency of Hy-1622 chloride synergistically with Span 80/85 to extract cerium ions with LSMs technology for emulsions in the oil phase is critical as it determines the stability, viscosity and mass transfer resistance of the resulting emulsion. Besides, Hy-1622 chloride was found as a new cationic surfactant that appeared in FTIR characterization and surfactant was found to speed up the permeability process and accelerate the extraction rate due to electrostatic interaction with the carrier.

Mathematical Modeling of Covid-19

Background The novel coronavirus (COVID-19) suddenly appeared in Wuhan, Hubei since December 2019, and quickly swept across China, then the whole world. Today, after more than 100 days of fighting against the virus, China's epidemic has been effectively controlled, but when we looking at the entire world, the novel coronavirus has rampaged globally, especially in the United States and many European countries. This paper mainly studies the impact of COVID-19 outbreaks at Hubei Province and the United States, fits the given data and predicts future trends. Methods Based on the theoretical basis of traditional differential equations and SIR infectious disease model1, and combined with the actual situation to improve the model. Hubei Province is modeled in different time periods, and the effects of birth rate and natural mortality on the model are analyzed. Since the birth rate and natural mortality in the United States in recent years cannot be found, the epidemic situation in the United States can only be analyzed based on the absence of births and natural deaths. Finally, we used Netlogo2 to establish a closed environment (Small World), and combined with known data to conduct simulation experiments on COVID-19 infection. Findings Through the analysis of given data through the SIR model, it is found that before the Chinese government has taken comprehensive measures to cure patients (before 10 February), the number of patients in Hubei Province will reach the peak at the end of February, and will gradually decline thereafter, and on 20 March, the epidemic will be effectively controlled in the future, which coincides with the fact that Wuhan closed the last mobile cabin hospital on 10 March. On the other hand, after the Chinese government tried its best to cure the patients (after 21 February), the number of patients continued to decline over time and will reach 0 in mid-April, which is also consistent with the actual data. According to the factors of birth and natural death, the sensitivity analysis of the above model found that when the epidemic situation is at its peak, it has little effect on the curve, but when the epidemic situation gradually flattens, it still has a certain effect on the trend of the curve. Finally, looking at the situation in the United States, due to the high transmission rate, the number of patients in the United States continues to rise and is expected to reach its maximum in mid-June. We also use Netlogo to simulate the environment in which the virus spread, and find that the general trend of the curves is also consistent with the actual curves. Interpretation The Chinese government has taken various measures to deal with the novel coronavirus pneumonia, including the establishment of two temporary hospitals and dozens of sheltered hospitals, the temporary transformation of university dormitories into isolation rooms345, the closure of Wuhan, the ban on the movement of people and so on. These measures have helped to reduce the spread of the virus and greatly increased the patient's cure rate. But the US government ’s actions are not as effective as China’s, not only because the government ’s actions are inappropriate and untimely, and the people’s opposition to isolation has not subsided. As a result, the virus has spread widely in the United States. More than one million people have been infected with the virus, and tens of thousands of people have died from COVID-196.

Statistical Analysis on the Influence of Flipped Classroom Teaching on Students’ Learning Effect During the Coronavirus Disease 2019 Epidemic

Currently, many educational practitioners do not agree on how flipped classroom affects students’ learning effect. In order to further explore the impact of flipped classroom on students’ learning effect, this paper conducts a quantitative analysis of some flipped classroom experimental and quasi-experimental studies systematically by means of meta-analysis method. The study finds that the random effect model shows that the combined effect is 0.373, reaching the statistical significance level, which indicates that flipped classroom has moderate positive effect on improving students' learning effect. There is no significant difference in the effect of flipped classroom on the learning effect of different subjects and stages, but the effect on primary school students is weaker. Significant differences in the effect on learning outcomes among different knowledge types have been found, and specifically, the flipped instruction is good for the study of practical knowledge, but has less influence on theoretical knowledge learning. Therefore, in the application of flipped classroom, it is necessary to pay attention to the characteristics of different learning objects and types of knowledge, and flipped classroom teaching cannot be used too much in primary school and the teaching of theoretical knowledge during the coronavirus disease 2019 epidemic.

Evaluation of Organic and Vermi Composts for Mass Culturing of Trichoderma Harzianum to be Used Against Soil-Borne Pathogen Sclerotium Rolfsii of Groundnut

The experiments were conducted in the fields of Plant Pathology Division, Bangladesh Agricultural Research Institute, Gazipur during 2016-17, 2017-18 and 2018-19 cropping years to evaluate the organic and vermi composts for mass culturing of biological control agent Trichoderma harzianum and toobserve the effect of formulated T.harzianum designated as Tricho-vermi-compost and Tricho-organic-compost as well as organic compost, vermi-compost and chemical fungicide Provax 200 WP against soil-borne pathogens, Sclerotium rolfsiiof groundnut causing foot and root rot/stem rot disease. The pathogen inoculated field soils were treated with Tricho-vermi-compost and Tricho-organic-compost, organic compost and vermi-compost 7 days before seed sowing where as seeds were treated with Provax 200 WP at the time of seed sowing. From this study it was revealed that all the treatments performed in reducing seedling mortality and increasing plant growth and yield of groundnut compared to control. Among the treatments, soil treatment with Tricho-vermi-compost and Tricho-organic-compost are the best treatments in reducing seedling mortality and increasing plant growth parameters and yield of groundnut which was significantly differed from the other treatments including control. Seed treatment with chemical fungicide Provax 200 WP and soil treatment with only vermin-compost and organic compost also promising treatments for management foot and root rot disease and increasing plant growth parameters as well as yield of groundnut compared to control.

Vineyard Clusters Monitored by Means of Litterbag-NIRS and Foliar-NIRS Spectroscopic Methods

There is currently a lack of rapid indirect analysis methods for the assessment of the effects of soil microbiota on vine production. Fifteen clusters of two Nebbiolo and Erbaluce varieties were identified in five vineyards belonging to a cooperative of winemakers in North West Italy, according to the differences in the NDVI index, as monitored by the Crop Monitoring OES system. The vineyards were surveyed in 2019 and the experimental monitoring of 75 vines was conducted in 2020. The first indirect method (Litterbag-NIRS) involved examining hay litterbags with a smart SCiOTM device. The average litterbag-NIR spectra of the clusters, as far as the yield is concerned, were closely fitted with the measured production yield, with an R2 cross-validated value of 0.91 in the Nebbiolo vines and 0.67 in the Erbaluce vines. The results in yield were accounted for by considering a few dominant variables in both vines, namely the microbic respiration of the soil and the crude protein of the litterbag (positive), opposed to the soil NO3--N and litterbag ADF (negative). The pruning wood was also closely correlated to the litterbag spectra. A second rapid method, foliar pH coupled with the NIR spectroscopy of the leaves, was then performed. The overall results predicted from the foliar NIRS were 0.73 for yield and 0.79 for the Canopy Cover. However, the most interesting result concerned the yield regressions on the foliar pH, which were clearly negative in both vines and of a similar amount: -5.15 kg/pH in Nebbiolo (R2 0.68) and -5.63 kg/pH (R2 0.23) in Erbaluce. Litterbag-NIRS, which shows a high predictive capacity, and foliar pH - with or without foliar-NIRS - are indirect and frugal methods that can be recommended for a rational assessment of the microbial soil fertility of vineyards.

Efficacy of Bat Guano on Growth and Yield of Some Hybrid Sunflower (Helianthus annus, L.) Cultivars in Sudan

Today; there is an increasing demand for fertilizers due to the intensive and extensive agricultural activity to meet the ever increasing demand for food and fiber by the rapid world population expansion. This study is mainly concern with the Bat Guano which is usually collected from the Caves. This experiment was conducted at the Farm of the College of Agriculture, University of Bahri, Alkadaro, Khartoum North-Sudan during the period from July to November, 2017 with the objective to investigate the efficacy of Bat Guano and Nitrogen fertilizer on growth and yield of Serena and Opera (Helianthusannus, L.) Hybrid Sunflower cultivars. The study was based on split-plot experiment in randomized complete block design (RCBD) with four (4) replications. Data pertaining Plant Height (cm), Leaf Number (LN), Leaf Area (LA), Leaf Area Index (LAI) and Seed Weight (SW) were studied. The said data were analyzed using Statistic 8- software. The results reflected significant increase in plant height (F= 6.05, P < 0.0001, leaf number (F=2.37, P<0.0388), leaf area (F= 7.44, P<0.001), leaf area index (F= 6.53, P < 0.0001) and seed weight of both Sunflower cultivars respectively due to application of Guano and Nitrogen fertilizer compared to the control. It also reflected non-significant difference between all studied traits as application of either Guano or Nitrogen fertilizer. Moreover, all studied characters of the two cultivars have similarly responded to the application of the two types of fertilizers. Further studies are recommended to assess the benefits from the use of Bat Guano as a fertilizer.

UV rays Decrease Foliar pH in Cress (Lepidium Sativum) and Modify NIR Spectrum

An experiment on watercress (Lepidium sativum) 42 d days after sowing was set up to evaluate the effect of UVAB radiation on foliar pH and on NIR tomoscopy. The effect of 8 h of UV exposure (about 500 µWcm-2) was clear, causing a reduction in the foliar pH of 6.0%, compared to the contemporary control group (5.22 vs. 5.56). When the treated leaves were returned to white light, their pH rose by 1.7% (5.31), reaching the same level as leaves grown outdoors (5.33). On the basis of the NIR spectra, the leaves of the plants grown outdoors were recognized 100%, that is, more than the control leaves (87%) and the plants treated with UV on the day of measurement (81%). However, the effect of the UV marking on the leaves is not permanent as, after the UV treatment, the leaves returned to white light were classified at 61%, which is a greater value than the 37% of overlapping between the two groups. The foliar pH was highly correlated with the NIR spectra, with R2 0.43. These results corroborate the hypothesis of the influence of the solar cycle on the variation of the pH of leaves in plants, which we believe may have played a major role in past agricultural pandemics. These results call for the introduction of the pH test for the evaluation of resistance to pathogens. From the environmental point of view, the foliar pH could be enlisted as an in vivo long-time indicator of the response of plants to climate changes.

The Feasibility of Enzyme Immunoassay Tests in the Absence of a Conventional Source of Electricity

Background of the Study African  countries are facing frequent blackout. Thus in sub Saharan region, due to frequent power cut, the laboratory professionals find sometimes difficulty to carry out earlier diverse diagnostic tests. Objective The  aim of this work is to evaluate the feasibility of enzyme immunoassay tests in the absence of a conventional source of electricity. Methods We  developed a battery-powered experimental device, which was then applied to diagnose measles. The samples included 45 sera randomly selected from non-haemolysed serum samples received and stored at the National Public Health Laboratory of Benin. The experimental device is composed of two devices (Devices 1 and 2). The Device 1 provided an average temperature of 34.47 °C, 20 min after starting. With Device 2 an average temperature of 20.32 °C is obtained 15 min after starting. Results With  the experimental device the same rate of measles antibody-positive sera (44.68%) was obtained as recorded from the test using the standard equipment of laboratory. The experimental device detected 18 negative and 8 intermediate results against respectively 19 and 7 by the standard equipment. The analysis of the results of both equipments shows a concordance rate of 93.33% with a kappa reproducibility coefficient of 0.89. Conclusion The  device conceived in our study is a simply equipment allowing the realization of the enzyme immunoassay tests, in this case the ELISA anti-measles test. The rate of concordance obtained shows that this device can be used with commercial kits and at temperatures close to those recommended by the manufacturer without altering the results.

The Interaction Model of Concentrated Solar Radiation with Materials

The paper analyzes approaches to modeling the processes of interaction of concentrated solar radiation with materials. The experimental results obtained on the synthesis of materials from a melt in a solar furnace are presented. The features of the interaction of concentrated solar radiation with materials are analyzed. The mechanisms are described and a model for the interaction of concentrated solar radiation with materials is created. A feasibility study is proposed for the technology of glass-based glass materials obtained on the Big Solar Furnace.

Effect of Drought and Salt Stress on Cereal Crop Plants and their Proteomic and Physiological Studies

The photosynthetic potential and underlying internal metabolism of a plant are some of the most commonly affected physiological functions as a direct consequence of stresses due to salt and water resulting in hindering plant growth and productivity. Under the influence of such detrimental stresses, a drastic alteration in a plant's osmotic requirements, hormonal production, shedding of leaves, and closure of stomata, along with a lessening in the diffusion and transportation of CO2 and H2O are commonly seen. This review unfolds with a description of the basic methodology involved in the proteomic analysis of various proteins involved in stress response along with a brief idea on identifying and obtaining a genomic sequence for proteomic studies. It then dives deep into understanding the impact of abiotic stresses such as salinity, drought and high temperatures on cereal crops such as rice and sorghum as well as the internal dynamics of tolerance mechanism unfolding during stresses have also been described. Extensive literature describing the proteomic and physiological responses to primary and secondary effects of salt stress in cereal crops emphasizing on ROS production and apoptosis, the role of osmolytes as ROS scavengers during osmotic stress and vacuolar antiporters in ionic stress along with the responses during drought stress such as the accumulation of LEA proteins and ABA-based signaling has been reviewed and critically discussed. The study also sheds light on some experimental proteomic studies conducted on the seedlings, root tissues, and shoots of rice cultivars.

Prevention of Aging and Improvement of Longevity and Life-Span in D-Galactose Induced Aging Rats After Treatment with the Biofield Energy Per Se and Biofield Treated Proprietary Test Formulation

The study was aimed to investigate the potential benefits of the Consciousness Energy Healing Treatment (the Trivedi Effect®) per se and Biofield Energy Healing treated novel test formulation in male Sprague Dawley rats for their antiaging activity by monitoring aging biomarkers such as brain-derived neurotrophic factor (BDNF), silent information regulator-1 (SIRT-1), and klotho protein. The test formulation was distributed into two parts. First part did not provide any Biofield Energy Treatment was denoted as the untreated sample, however the second part was received Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi and defined as the Biofield Energy Treated sample. In this experiment, nine groups (n=10) were assigned, in which four were preventive maintenance groups. Among them, three groups of animals were also received Biofield Energy Healing Treatment per se (at day -15). BDNF was significantly increased by 25.83%, 19.35%, and 14.67% in the Biofield Energy Treated test formulation (G5), Biofield Energy Treatment per se at day -15 (G6), and Biofield Energy Treatment per se to animals plus Biofield Treated test formulation from day -15 (G8), respectively as compared to the disease control (G2) group. Moreover, expression of SIRT-1 protein was increased by 14.63% in the G5 group than the untreated test formulation (G4) group. Additionally, SIRT-1 activity was increased by 39.7%, 32.5%, 15.9%, and 136% in the G6, Biofield Energy Treated test formulation at day -15 (G7), G8, and Biofield Treatment per se (day -15) to animals plus untreated test formulation (G9) groups, respectively than the G4 group, while it was increased by 57.3% in the G9 group as compared to the G2 group. Besides, Klotho protein in kidney homogenate was significantly increased by 16.67% in the G5 group as compared to the G2 group. Altogether, the results showed a significant improvement of longevity mediators and antiaging biomarkers in the preventive maintenance groups. Therefore, results envisaged the significant slowdown of aging-related disorders and other complications in the preventive Biofield Energy Treatment group per se and/or Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) comparatively with the disease control group and could be utilized against various aging-related disorders like Alzheimer's disease, hypertension, osteoporosis, cataracts, type 2 diabetes, cancer, etc. along with it could be used to extend the life-span, stress and immune-related disorders.

Effects of Selected Secondary Metabolites in Leaf Extract of Jatropha Tanjorensis on Some Gonadal Hormones in Male Wistar Rats

Background and Objective The use of medicinal plants in industrialized societies for extraction and development of many drugs and other chemotherapeutics and traditionally for herbal remedies has increased in recent times. Plant–based medicine is essential in health care services with about 80% global population relying on it because of its cheap source and availability. Jatropha tanjorensis is one such plant used by males and females of childbearing age for treatment of reproductive problems such as infertility. Literature on isolation and characterization of the secondary metabolites in this plant may not be common. Against this backdrop, this research work was carried out to isolate, characterize and determine the effects of J. tanjorensis on the gonadal hormones of male wistar rats. Materials and Methods The secondary metabolites were isolated, characterized, and identified using nuclear magnetic resonance. The experiment was conducted using 25 male wistar rats weighing between 180-200 g randomized into 5 groups, 3 controls and 2 treatment groups of 5 rats each. The treatment groups received 25 mg/kg body weight of phytol and lupeol orally by gastric lavage for 14 days. The animals were anaesthetized and blood samples collected for hormonal assay. Result The experimental data was analyzed using one-way analysis of variance (ANOVA) with Statistical Package for Social Sciences (SPSS) version 17.0, while the post hoc test assessed using Duncan Multiple Range Test at p ≥ 0.05. There was a significant decrease (p ˂ 0.05) in the levels of FSH, LH and TST in the treatment groups when compared to the control groups. The motility and sperm count decrease significantly (p ˂ 0.05) when treatment groups were compared to the control animals. The secondary metabolites, phytol and lupeol present in the leaf extract of Jatropha tanjorensis were responsible for the decrease in some of the gonadal hormones studied.

Efficacy of Phytochemical Constituents of Castor Essential oil Towards the Mucor-Mycotic Mold Cunninghamella Bertholletiae

The aim of this experiment is to study the efficacy of phytochemical constituents of Castor essential oil towards the mucor-mycotic mold Cunninghamella bertholletiae.The standard chemical analytical methods were used for the rapid study of the phytochemical constituents responsible for the antimicrobial efficacy of the procured castor essential oil. The standard antimicrobial assay technique employed to study the comparative values of the efficacy of the procured castor essential oil with that of the standard antifungal chemical agents against the clinical isolates obtained from the immune suppressed patients samples of Cunninghamella bertholletia mold mucor-mycotic infections. The best susceptibility values recorded in the standard antifungal agents against the clinical isolates of Cunninghamella bertholletiae was with Amphotericin B showing the average zone of inhibition diameter of 20.66 mm with the average MIC value of, 1.66 (µ/ml) but the antimicrobial assay results for the Castor essential oil showed better values with an average disc diffusion of 22.44mm zone of inhibition diameter with average MIC value of 1.72 µ/ml .This study has shown that the phytochemical compounds present in the Castor essential oil proves to be more an effective alternative antifungal substance towards the clinical isolates of Cunninghamella bertholletiae.

The Impact of Combining Nutrition Education with Active Choice on the Fruit and Vegetable Consumption Among Second Grade Students

Daily intake of fruits and vegetables provides the basis for healthy nutrition. Yet low consumption of fruits and vegetables (FV) persists among school-aged children. Framed by the Social Cognitive Theory, this study aimed to determine the effectiveness of nutrition lessons combined with an active choice intervention on children’s FV consumption. Using a quasi-experimental design, 89 second graders were assigned to groups. Students in the experimental group (n=46) received four nutrition lessons combined with nine active choice sessions, while those in the control group (n=43) received active choice sessions only. Responses to pre- and post-intervention surveys that inquired FV knowledge and consumption were compared within and between groups using independent and paired t-tests. Empowered by improved knowledge, self-efficacy and the positive environment created through nutrition lessons and active choice, the experimental group showed improvement in consumption behavior compared to the control group at post-intervention: bringing FV to school and finish eating (69.7 vs. 51.2, p=.05 for fruits; 43.5 vs. 39.5, p=.41 for vegetables); like choosing FV (84.8 vs. 65.1, p=.01); like to eat more FV (80.4 vs. 62.8, p=.16); FV are healthy (100 vs. 95.3, p=.14). The magnitude of improvement is small yet consistent in every aspect of outcome measures. Combining nutrition education with the active choice component showed potential for a larger impact on behavior change among study participants. Parental support and community involvement could enhance the effectiveness of nutrition education in schools.

The Study of Coexistance of Superconductivity and Spin Glass in Fe Pnictide(Fe1+YSeXTe1−X)

Superconductivity and magnetism were previously thought as incompatible until the discovery of some rare earth ternary compounds that shows the coexistence of superconductivity and magnetism. In some of the recently discovered iron based layered superconductors superconductivity and diamagnetic order system are coexist. That occurs in only 11 and 122 family. The present works we examine the possibility of coexistence of superconductivity and disorder of magnetic spin is called spin glass when freeze the system that can show the superconductivity and spin glass coexist. In this present work we can examine the possibility of coexistence of superconductivity and spin glass in detailed 11 family of Fe1+ySexTe1−x compound. We show that spin glass like behavior is present in FST for x = 0.1 - 0.15 we present evidence form magnetization measurement and characterized the short-range order with neutron scattering. One of our main results is that the short-range order is structural as well as magnetic order. The factor of magnetic order exchange in long range depend on temperature, pressure, number of doping and other external factor discussed it. We found mathematical expression for superconductor transition TC, spin glass temperature TgSusceptibility x(q), and retardation time τ using for born approximation and digamma function depend on wave vector(q) and cut off frequency(ω) in the region coexistence of superconductivity and spin glass in Fe1+ySexTe1−xcompound. Aim: To improve the coexistence of superconductivity and spin glass and to examine the associated Factor of electron doping and temperature in experimental and theoretical aspect of their coexistence.

Response of Some Sunflower (Helianthus annuusL) Hybrid Cultivars as Influenced by Argel (Solenostemmaargel, Del. Hayne) and Nitrogen Under Irrigation in Sudan

A field experiment was conducted during 2017/2018 to assess the effects of Argel (Solenostemmaargel, Del. Hayne) and Nitrogen fertilizer on the performance of two Sunflower Hybrid cultivars, with the objectives to exploit an easy, economic, and accessible organic fertilizer, the Argel in the phase of the continuous increasing prices of chemical fertilizers.. This experiment was laid out on spilt-split-plot experiment in Randomized Complete Block Design (RCBD) with four replications. Observations were taken on some agronomic traits (Plant height (m), Leaf area (LA), leaf Number (NL), Leaf Area Index (LAI) and Mean Seed Weight). The results revealed that the application of Argel and Nitrogen fertilizer have significantly increased the leaf Area (F= 7.22, P < 0.001), Plant Height (F=2.68, P < 0.2001), Leaf Number (F= 3.90, P < 0.0024), Leaf Area Index (F= 3.83, P < 0.0026) and Mean Seed Weight of both Serena and Opera cultivars respectively compared to the control. However, the different treatments of Argel and Nitrogen reflected variable degree of increase for the studied parameters. The study concludes that Argel is very promising, therefore a further study with different levels of Argel including other plant parameters is recommended.

Acute Toxicity of Clarias Gariepinus Fingerlings Exposed to 2,4-D Dimethylamine Salt

This study investigated the acute toxicity of Clariasgariepinusfingerlings. The fingerlings of Clariasgariepinuswere acclimatized for 1 week before the range-finding test was carried out at varying concentrations. Sublethal concentration (viz: 0.00ppm, 10.80 ppm, 18.00 ppm, 25.20 ppm, 32.40 ppm and 39.60 ppm of the 2,4-D Dimethylamine salt) were made in a rectangular aquarium. Each experimental concentration was carried out in triplicate with 10 fish each. The media were renewed at every 24 hours throughout the experimental duration viz: 96 hours. When the fish were introduced into the aquarium containing the toxicants, they exhibited some behavioural changes including opercular movement, air gulping and irregular swimming pattern. The mortality rate significantly increased as the concentration of the 2,4-D Dimethylamine salt increased for each of the exposure duration. The LC50 values at 24, 48, 72 and 96 were 86.15 ppm, 36.28 ppm, 18.72 ppm and 15.68 ppm, respectively. From the findings of this study, there is a need for exercise caution in the use of 2,4-D Dimethylamine salt based herbicides close to the aquatic ecosystem.

Histological Studies of Effect of Dichlorvos on Hypothalamic-Pituitary-Testicular Axis Following the Administration of Mimosa Pudica Extract

Plants have played a significant role in maintaining human health and improving the quality of human life. Therefore, this present study seeks to evaluate the role of its alkaloid fraction in the hypothalamic-pituitary-testicular axis after dichlorvos poisoning in mice. A total of 25 male mice, weighing approximately 20-30 g, were randomly assigned into 5 groups of 5 mice each (Groups A, B, C, D and E). Mice in groups A served as control and received normal saline while groups B, C and E. received 5 mg/kg of dichlorvos daily for 14 days. Mice in group D received 200mg/ kg of Alkaloid extract of Mimosa pudica (AEMM) for 14 days. At the end of the experiment, all animals were sacrificed and Hypothalamus, Pituittary and Testes were excised for histological process. Sperm from cauda epididymidal duct on one side was collected and used for spermatological studies and data were expressed as mean± SEM. Significant difference was set at p<0.05. Results showed significance difference (p<0.05) in sperm motility, sperm life death ratio groups when compared with their controls and no significance difference in sperm volume when compared with control. Histological studies reveal distortion in hypothalamuss, pituitary and testes in dichlorvos administration group compared with control rats while mimosa showed a ameliorative effect when co-administered with dichlorvos. In conclusion, the alkaloids from Mimosa (AEM) leaves enhanced the released of gonadotropins by the cordial action of the hypothalamus-pituitary axis for the reversed of Dichlorvos effect.

A Study on Redox Potential of Phytochemicals and their Impact on DNA

The phytochemicals are endowed with antioxidant activities because of the presence of plentiful polyphenols and many other phytochemicals. However, some recent reports have suggested that phytochemicals from certain plant species exhibit DNA damaging properties mainly due to presence of alkaloids. In the present study, pBR322, Salmonella typhi DNA, insect DNA and human DNA were treated with hexanolic extract of Argemone mexicana and Thevesia peruviana leaves to assess their DNA damaging abilities. Another set of experiments was carried out using the methanolic extracts of Citrus lemon leaves to assess their DNA protecting abilities from damage of DNA by extracts of A. mexicana and T. peruviana at 150000 ppm for all DNAs used. The results indicated that the leaves extract of A. mexicanaand T. peruviana demonstrated significant DNA damaging potential at higher concentrations. In contrast, the extracts from C. limonat 15000 ppm showed maximum DNA protective properties for all DNAs used.

Formulation of Isohydricity Conditions in Acid-Base systems - A Review

The isohydricity conditions are formulated for D+T systems composed of titrand D and titrant T, mixed according to titrimetric mode; only acid-base equilibria are involved there. The original method of dissociation constants determination, based on the isohydricity principle, is presented and confirmed experimentally. The pH titrations in the system of isohydric solutions are also put in context with conductometric titrations.

Reproductive Parameters of Three Populations of Oreochromis Niloticus (Linnaeus, 1758) in the Sudano-Guinean Altitude Zone of Cameroon

In order to contribute to the improvement of aquaculture production, reproductive parameters of three populations of Oreochromis niloticus of Cameroon were studied between March and May 2019 at the research station of Foumban. At this effect, a total of 81 parents (27 males and 51 females with respective weight 124 ± 6g and 144 ± 5​​g) coming from three hydrogeographicals origins (Niger, Sanaga and IRAD Station) were randomly distributed in triplicate in nine concrete tanks of a m² each with a sex ratio of one male for two females (1♂/2♀). Throughout the test, six females carrying eggs in the oral cavity were collected in each population and 45 days after the start of the trial, all the offspring were collected and the adults sacrificed. At the end of experiment, the IRAD population presented highest significant values (p ≤ 0.05) regardless of the performances considered. The values of the gonado-somatic and the gonado-metric characteristics significantly lower (p ≤ 0.05) were obtained in Niger population (GSR = 0.04 ± 0.02, GSI = 0.05 ± 0.03 and GMR=0.19 ± 0.08;GMI= 0.24±0.07). Considering sex, males presented the lowest significant values (p ≤ 0.05) for all the characteristics studied (GSR = 0.04±0.01, GSI = 0.04±0.01 and GMR= 0.23±0.08;GMI= 0.29±0.09).

Auricularia Polytricha (Mushroom) Regulates Testicular DNA Expression and Oxidative Stress Markers of Streptozotocin-Induced Diabetic Male Wistar Rat

Local Nigerian men have been using AuriculariaPolytricha as a treatment for sexual dysfunction without supporting evidence from scientific experiments. This study was to investigate the effect of ethanolic extract of A. Polytricha on testicular DNA expression and some oxidative stress markers using STZ-Induced diabetic rats as a model. The experiment included six groups, Group A (Normal Control, treated with normal saline), Group B (treated with 65mg/kg.bw of STZ), Groups C, D, and E (treated with 250mg/kg.bw, 500mg/kg.bw, 1000mg/kg.bw AP after inducing diabetics), and Group F (treated with 40mg/kg.bw metformin after inducing diabetics). The experiment lasted for 35 days. After termination of the experiment, Fuelgen nuclear reaction was used for DNA demonstration to assess testicular DNA distribution while serum Superoxide Dimutase (SOD), Catalase and Melondialdehyde where evaluated using reagent based antioxidant enzyme assay. Results reveals that SOD and Melondialdehyde activities were remarkably (p<0.05) higher in diabetic control animals when compared with the normal control group. Values in Groups C, D and F that were administered with 250, 500mg/kg.bw A. polytricha and metformin respectively were also significantly (p<0.05) increased when compared with the normal control group. However, diabetic animals placed on 1000mg/kg.bw A. polytrichadid not show any statistical significance in comparison with normal control group but was remarkably (p<0.01) decreased when compared to the diabetic group that received low dose A. polytricha, an indication that the reversal is dose dependent. Catalase concentration in diabetic control animals was remarkably (p<0.05) higher when compared to the normal control but was not significantly (p<0.05) different in groups D (DM+500mg/kg.bw A. polytricha) and E (DM+1000mg/kg.bw A. polytricha) when compared with the normal control group. Diabetic control animals showed reduced magenta colour intensity of DNA and increased clustering and cross linking of DNA strands when compared with the normal control. However the degree of cross link in DNA strands was reduced in the diabetic animals placed on 1000mg/kg.bw A. polytrichawhen compared with the diabetic control group. Reversal in DNA damage and values of serum oxidative stress markers following administration of graded doses of A. polytricha could be attributed to essential phytochemical and therapeutic constituents in A. polytricha like polyphenol and flavonoid which can be found useful in prevention and treatment of diabetes induced testicular dysfunction. In summary, AP can contribute to a reversal in DNA damage and levels of serum oxidative stress markers in treating diabetes-induced testicular dysfunction.

The Effect of 2,4 Dimethylamine salt on the Blood, Liver and Muscle of Oryclotagus Cuniculus

Aminoforce containing 720g/l of 2,4-dimethylamine salt induced changes on some enzymes and electrolytes in the male Oryclotagus cuniculus (New Zealand rabbit) were assayed. The organisms were exposed to varying sub-lethal concentrations of the toxicant (720g/l). The concentrations were prepared by pipetting 0.4mls, 0.8mls and 0.12mls making it up to 1.5L clean water in a metal container to make 2.0 mgl-1, 4.0 mgl-1 and 6.0 mgl-1. Aspartate amino transferase (AST), Alanine amino transferase (ALT) and Acid phosphatase (ACP) were assayed in the liver and blood. Results showed that aspartate amino transferase values in the liver and blood were significant (p<0.05) across the concentration of the toxicants. Aspartate amino transferase increased as the concentration of the toxicant increased in the liver, and decreased as the toxicant concentration increased in the blood. Alanine amino transferase in the blood and liver were akin to AST while ACP values increased in the blood and decreased in the liver as the concentration of the toxicant increased. Electrolytes (Sodium (Na+), potassium (K+) and magnesium (Mg2+) ions) showed statistical deviation across the various concentration of the toxicants. Chloride ion values stabilized in the experimental group being not significantly different (p>0.05) across the various concentration of the toxicants. From the study, AST, ALT and ACP are suitable biomarkers for showing sub-lethal effect of aminoforce on Oryclotagus cuniculus. The effects recorded clearly unveiled the potential effect of this xenobiotics on Oryclotagus cuniculus. Therefore, exposure of Oryclotagus cuniculus to this toxicant will affect the organism’s physiological responses and over prolong period of time it could lead to death. Additionally, via food chain man may be affected. The use of this toxicant close to rabbittory should be done with utmost caution.

Analysis and Forecast Based on the Kinetic Equation for Changing the Numerical Composition of Living Systems

The possibility of applying the kinetic theory of aging of biological species published earlier by the authors of this work to assess and predict changes in the number of specific populations is evaluated. The populations of the USA, China and Russia, as well as the population of mice observed in the experiment "mouse paradise" of the American scientist John Calhoun are considered. To this end, a historically consistent analysis of the main previously proposed multi-scenario mathematical models describing demographic data and predicting the dynamics of the population was performed. The results of these models show a decrease in the population growth rate, a tendency toward a limit with an increase in historical time, the achievement of such a limit in some developed countries with a relatively high level of social security, a subsequent decrease in the number and further uncertainty of the final population outlook in the distant future. In addition, these models made it possible to establish that the observed population growth in developed countries is unambiguously accompanied by its aging - a relative predominant increase in the number of elderly people compared to the number of the younger generation (people are aging, the population of countries is aging). In this work, the assumption was made and confirmed that the dynamics of the aging of the population of the countries of the World corresponds to the dynamics of aging of a person of one generation and is mathematically described by the differential equation of the kinetic theory of aging of living systems of the same type with close values of the parameters. The biophysical meaning of the parameters of the kinetic equation reflects G. Selye's concept of the determining role of stress in human life and populations. An analysis of the changes in the numbers of the considered populations of humans and mice at various stages of their development is qualitatively commented on from the standpoint of comparative tension according to G. Selye. To assess the degree of aging of a biological object of one population in kinetic theory, the probability of death during life is selected as an indicator of aging. In this work, the probability of reaching the maximum population size was chosen as an indicator of the aging of a biological object of various populations. The published literature predicts various options for changing the population after reaching a maximum - maintaining the reached maximum level and decreasing to a certain limit, less than the maximum achieved. In this paper, based on an analysis of its results and an analogy with the complete degeneration of mice in the “mouse paradise” experiment, a conclusion is drawn about a hypothetically possible third variant of the limiting decrease in the population - its complete degeneration.

The Effect of Aqueous Extract of Ocimum gratissium (Linn) on 1, 2 - Dimethyl Hydrazine induced Colon Cancer in Male Wistar Rats

Background Colon cancer is one of the leading causes of cancer death world-wide. There is a steady increase in incidence over the past four decades in developing countries. This has been partly attributed to increasingly low intake of vegetables among other causes. Aims Therefore this study aims to evaluate the protective effect of aqueous extract of Ocimumgratissimum (OG) leaves (a staple vegetable) on experimental model of colon carcinogenesis induced with 1, 2 Dimethylhydrazine (DMH). This is compared with celecoxib (a cyclooxygenase-2 inhibitor) which is used in the chemoprevention of colon cancer. Methods Sixty adult male Wistar rats were randomly divided into six groups: A to F, n=10. Group A was the normal control, Group B was given only DMH weekly for 16 weeks,Groups C, D and E were given graded doses of OG for two weeks prior to cancer induction by DMH. After which both OG and DMH were given for 16weeks. Group F received celecoxib daily for two weeks prior to cancer induction. Colonic wall was analysed grossly, histologically and biochemically. The induced lesions were staged investigated and staged using Duke’s Staging method. Results The result showed tumour incidence in groups B and C while no evidence of primary colonic tumour was observed in groups A, D, E and F. There was a dose dependent increase in the goblet cell count in the groups treated with OG with group E being statistically higher than group F. There was a significant reduction in collagen staining intensity (F = 129.74, p < 0.0001) for the colonic wall in group B when compared to other groups. There was a decreased nucleo-cytoplasmic ratio in groups C, D, E and F when compared to group B. There was a significant increase in the concentration of nitric oxide and prostaglandin E2in group B when compared to other groups D, E and F. Conclusion In conclusion, this research showed a protective effect of Ocimumgratissimum leaves on 1, 2-dimethylhydrazine-induced colon cancer which further corroborated its ethno-medicinal use. 

Arbuscular Mycorrhizal Biofertilizers Sources in the Potato (Solanum Tuberosum) Plant show Interactions with Cultivars on Yield and Litter-bags Spectral Features

Four strains of Arbuscular Mycorrhizal (AM) biofertilizer fungi, combined with two potato cultivars, were in-field tested in a four-replicate arrangement in a factorial experiment. As far as general combinability is concerned, cv. Agria was more responsive to different inocula (yield +5.56%, P 0.02) and to  two strains in particular (+8%). On the other hand, the results with Innovator, a cultivar that yields 33% less than Agria, showed a significant reduction in the number of tubers for three AM strains, thus proving a clear genetic Biofertilizer * Cultivar interaction. The study of hay litter-bags has shown a high NIR spectral fingerprint for the Cultivar factor (81%), while the Inoculation factor showed a higher spectral fingerprint in Agria (76%) than in Innovator (65%). The Substrate Induced Respiration predicted from the NIR-SCiO spectra of the litter-bags was significantly increased after inoculation (+6.3%, P 0.04), but appeared lower for Agria (-5.4%) vs. Innovator (P 0.05), with a non-significant interaction. The obtained results show that the adaptation of the AM strains to the genetics of potato cultivars  is a first step toward reducing chemical inputs, with consequent benefits for the environment, but without an excessive reduction in yield. The litter-bag technique can therefore be recommended for a simplified monitoring of the complicated plant-mycorrhizosphere relationship. 

Study of Antioxidant and Membrane Resistant Peculiarities of a New Cyan Containing Lactone in Membranes of Hepatocytes with Sarcoma-45

The antioxidant and membrane resistant peculiarities of a new derivative (2-cyan-3,4,4-trymethil-2-buten-4-olyd - CTBO) of cyan containing unsaturated lactones have been studied in membranes of hepatocytes with Sarcoma-45 1. The results of our previous research 123 showed significant changes of phospholipid (PL) exchange in hepatocytes of microsomal membranes at experimental animals vaccinated with Sarcoma-45 tumor strain. It is manifested in significant changes of quantitative and qualitative contents of membrane phospholipids separate fractions, increase of cytotoxic lysophospholipids (LPCs), phosphatidylinositol (PI) and phosphatidic acid (PA) levels, significant decrease of phosphatitylcholines (PC) and sphingomyeline (SP) contents, statistically significant changes of PL/PL ratio, peroxidation ratio intensity, dramatic increase of phospholipase A2 (PLA2)activity, quantitative and qualitative changes of adenyl nucleotides, as well as disorders of adenosine triphosphatase (ATPase) system activity 34567.

Performance of New and Old Short-Seasoned Arachis Hypogea (Groundnut) Varieties Under Same Agronomic Practices

Performance of three newly released short-seasoned (Nsinjiro, Chitala and JL11) were evaluated against old (Nyanda, Illanda and Tern) groundnuts varieties under same agronomic practices. A field experiment laid in a randomized complete block design (RCBD) with three replicates was done. Varieties were evaluated for days to 50% emergence, 50% flowering, days to physiological maturity, pod and seed yield, and shelling percentage. There were no significant difference in the days to 50% emergence among all varieties but significant difference (p ˂ 0.05) were observed on days to 50% flowering. JL11 and Tern took shortest (90 days) and longest (120 days) time to physiological maturity respectively. Chitala had highest (3.804t/ha) and Tern had lowest (3.020t/ha) seed yield. Jl11 had highest (83%) and Nyanda least (68%) shelling percentage. Results showed that the new varieties out-performed the old varieties in all measured parameters. Therefore, resource constrained farmers may safely opt for the new short-seasoned varieties over the old ones.